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Decreased syndecan-2 expression correlates with trichostatin-A induced-morphological changes and reduced tumorigenic activity in colon carcinoma cells

Title
Decreased syndecan-2 expression correlates with trichostatin-A induced-morphological changes and reduced tumorigenic activity in colon carcinoma cells
Authors
Kim Y.Park H.Lim Y.Han I.Ho J.K.Woods A.Oh E.-S.
Ewha Authors
오억수
SCOPUS Author ID
오억수scopus
Issue Date
2003
Journal Title
Oncogene
ISSN
0950-9232JCR Link
Citation
vol. 22, no. 6, pp. 826 - 830
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The inhibition of histone deacetylase activity is known to induce morphological changes of transformed cells. In this study, we investigated the effect of the specific HDAC inhibitor, trichostatin A (TSA), on colon carcinoma cell lines. Treatment of human colorectal carcinoma cells, KM1214 and KM12SM, with TSA induced distinct morphological changes. Both cell lines, which normally piled up in layers without clear boundary, became more flattened, and formed monolayers with evident boundaries between cells, with concomitant increased actin filament organization. Cell-cell interaction was not affected much, based on expression level, membrane localization, and interaction of E-cadherin with β-catenin. In contrast, syndecan-2 expression was dramatically reduced and it was correlated with the morphological changes of colon carcinoma cells. Consistently, downregulation of syndecan-2 expression by antisense cDNA clearly mimicked the morphological changes in KM12SM and reduced anchorage-independent growth of colon cancer cells. All these results indicate that reduced syndecan-2 expression correlates with TSA-induced morphological changes and reduced tumorigenic activity in colon carcinoma cells.
DOI
10.1038/sj.onc.1206068
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자연과학대학 > 생명과학전공 > Journal papers
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