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Recovery of poly(3-hydroxybutyrate) from high cell density culture of Ralstonia eutropha by direct addition of sodium dodecyl sulfate

Title
Recovery of poly(3-hydroxybutyrate) from high cell density culture of Ralstonia eutropha by direct addition of sodium dodecyl sulfate
Authors
Kim M.Cho K.-S.Ryu H.W.Lee E.G.Chang Y.K.
Ewha Authors
조경숙
SCOPUS Author ID
조경숙scopus
Issue Date
2003
Journal Title
Biotechnology Letters
ISSN
0141-5492JCR Link
Citation
vol. 25, no. 1, pp. 55 - 59
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
A simple and effective method for the recovery poly(3-hydroxybutyrate) [P(3HB)] directly from high cell density culture broth with no pretreatment steps has been developed. This method consists of direct addition of sodium dodecyl sulfate (SDS) to the culture broth, shaking, heat treatment, and washing steps. When the SDS/biomass ratio was higher than 0.4, the purity of recovered P(3HB) was over 95% for various cell concentrations of 50-300 g dry cell 1 -1, with the highest value of 97%. The recovery of P(3HB) was over 90% regardless of cell concentration and SDS dosage (SDS/biomass ratios, 0.1-0.7). One g SDS digests 0.72 g non-P(3HB) cell materials. The reduction in molecular weight, due to degradation of P(3HB) by SDS, was negligible.
DOI
10.1023/A:1021734216612
Appears in Collections:
엘텍공과대학 > 환경공학전공 > Journal papers
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