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Creatine Kinase B Is a Target Molecule of Reactive Oxygen Species in Cervical Cancer

Title
Creatine Kinase B Is a Target Molecule of Reactive Oxygen Species in Cervical Cancer
Authors
Choi H.Park C.S.Kim B.G.Cho J.W.Park J.-B.Bae Y.S.Bae D.S.
Ewha Authors
배윤수
SCOPUS Author ID
배윤수scopus
Issue Date
2001
Journal Title
Molecules and Cells
ISSN
1016-8478JCR Link
Citation
vol. 12, no. 3, pp. 412 - 417
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
Recently, a procedure for detecting ROS-sensitive proteins that contain active cysteine residues was developed. The method is based on the fact that biotin-conjugated iodoacetamide (BIAM) and ROS competitively and selectively react with the active cysteine residues in ROS-sensitive proteins. To investigate the role of ROS in cervical cancer, BIAM labeling on cytosolic proteins in normal and cancer tissues was performed, respectively. The BIAM labeling proteins are separated by 2-dimensional electrophoresis, and then identified by MALDI-TOF mass analysis. ROS-sensitive protein is identified as creatine kinase B containing cysteine residue in active center. Activity of creatine kinase B in normal tissue is higher than that of oxidized form in cervical cancer tissues. The result suggests that ROS play an important role in metabolic regulation in cervical cancer cells. However, molecular mechanisms that ROS and creatine kinase B are integrated into a physiological signal leading to the cellular transformation remain to be elucidated.
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자연과학대학 > 생명과학전공 > Journal papers
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