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Fluorescence lifetime distribution of single molecules undergoing Förster energy transfer

Title
Fluorescence lifetime distribution of single molecules undergoing Förster energy transfer
Authors
Lee M.Tang J.Hochstrasser R.M.
Ewha Authors
이민영
SCOPUS Author ID
이민영scopus
Issue Date
2001
Journal Title
Chemical Physics Letters
ISSN
0009-2614JCR Link
Citation
vol. 344, no. 5-6, pp. 501 - 508
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The Förster energy transfer process of a donor-acceptor system was investigated at the single-molecule level as well as in bulk by confocal fluorescence lifetime microscopy. While the fluorescence decay of bulk tetramethylrhodamine ethyl ester (TMR) was nonexponential, the single TMR molecules exhibit single exponential decay in PMMA in the absence or presence of azulene. The lifetime distribution of single TMR molecules undergoing fluorescence energy transfer shows some deviation from that deduced from the Förster equation. Although the bulk data are well fitted by two exponentials, the measured distribution shows no physical basis for that assumption. © 2001 Elsevier Science B.V.
DOI
10.1016/S0009-2614(01)00862-4
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자연과학대학 > 화학·나노과학전공 > Journal papers
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