View : 36 Download: 3

Cell cycling status of human cord blood CD34+ cells during ex vivo expansion is related to the level of very late antigen expression

Title
Cell cycling status of human cord blood CD34+ cells during ex vivo expansion is related to the level of very late antigen expression
Authors
Seoh J.-Y.Park H.-Y.Chung W.-S.Kim S.-C.Hahn M.-J.Kim K.-H.Shin H.-Y.Ahn H.-S.Park K.-W.Ryu K.-H.
Ewha Authors
박혜영서주영김경효김승철정화순유경하
SCOPUS Author ID
박혜영scopus; 서주영scopus; 김경효scopus; 김승철scopus; 정화순scopus; 유경하scopus
Issue Date
2001
Journal Title
Journal of Korean Medical Science
ISSN
1011-8934JCR Link
Citation
vol. 16, no. 1, pp. 20 - 24
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
Very late antigen-4 (VLA-4), which binds to the extracellular matrix protein fibronectin, is an integrin molecule known to be modulated during mobilization of CD34+ cells, and to be involved in signaling the mobilization stimuli. On the hypothesis that cell cycling status might be different depending on the level of VLA-4 expression, we investigated the DNA contents of human cord blood CD34+ cells during ex vivo expansion by recombinant human thrombopoietin and flt3-ligand with simultaneous measurement of surface VLA-4 at the 1st and 4th week. During this ex vivo expansion, expression of VLA-4 increased and almost all cells became VLA-4+ until the 4th day of culture. Expression of VLA-4 was maintained in the major population of the cultured cells until the 4th week. The cells in S/G2/M phase were greater in number in VLA-4 high fraction than in VLA-4 low fraction (n=4, p<.001). Furthermore, the fraction of cells in S/G2/M phase increased as the expression of VLA-4 became higher. These results suggest that cord blood CD34+ cells expressing high levels of VLA-4 have more proliferative activities. Copyright © The Korean Academy of Medical Sciences.
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
Files in This Item:
Cell cycling status of human cord blood CD34+ cells during ex vivo expansion is related to the level of very late antigen expression.pdf(728.02 kB)Download
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE