Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이준승 | * |
dc.date.accessioned | 2016-08-28T11:08:21Z | - |
dc.date.available | 2016-08-28T11:08:21Z | - |
dc.date.issued | 1999 | * |
dc.identifier.issn | 0167-4412 | * |
dc.identifier.other | OAK-333 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/218584 | - |
dc.description.abstract | Indole-3-acetic acid (IAA) markedly increased ethylene production by inducing the expression of three 1-aminocyclopropane-1-carboxylate (ACC) synthase cDNAs (pVR-ACS1, pVR-ACS6 and pVR-ACS7) in mung bean hypocotyls. Results from nuclear run-on transcription assay and RNA gel blot studies revealed that all three genes were transcriptionally active displaying unique patterns of induction by IAA and various hormones in etiolated hypocotyls. Particularly, 24-epibrassinolide (BR), an active brassinosteroid, specifically enhanced the expression of VR-ACS7 by a distinct temporal induction mechanism compared to that of IAA. In addition, BR synergistically increased the IAA-induced VR-ACS6 and VR-ACS7 transcript levels, while it effectively abolished both the IAA- and kinetin-induced accumulation of VR-ACS1 mRNA. In light-grown plants, VR-ACS1 was induced by IAA in roots, and VR-ACS6 in epicotyls. IAA- and BR-treatments were not able to increase the VR-ACS7 transcript in the light-grown tissues. These results indicate that the expression of ACC synthase multigene family is regulated by complex hormonal and developmental networks in a gene- and tissue-specific manner in mung bean plants. The VR-ACS7 gene was isolated, and chimeric fusion between the 2.4 kb 5'-upstream region and the β-glucuronidase (GUS) reporter gene was constructed and introduced into Nicotiana tabacura. Analysis of transgenic tobacco plants revealed the VR-ACS7 promoter-driven GUS activity at a highly localized region of the hypocotyl-root junction of control seedlings, while a marked induction of GUS activity was detected only in the hypocotyl region of the IAA-treated transgenic seedlings where rapid cell elongation occurs. Although there was a modest synergistic effect of BR on the IAA-induced GUS activity, BR alone failed to increase the GUS activity, suggesting that induction of VR-ACS7 occurs via separate signaling pathways in response to IAA and BR. A scheme of the multiple regulatory pathways for the expression of ACC synthase multigene family by auxin and BR is presented. | * |
dc.language | English | * |
dc.title | Auxin and brassinosteroid differentially regulate the expression of three members of the 1-aminocyclopropane-1-carboxylate synthase gene family in mung bean (Vigna radiata L.) | * |
dc.type | Article | * |
dc.relation.issue | 4 | * |
dc.relation.volume | 41 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 443 | * |
dc.relation.lastpage | 454 | * |
dc.relation.journaltitle | Plant Molecular Biology | * |
dc.identifier.doi | 10.1023/A:1006372612574 | * |
dc.identifier.wosid | WOS:000083831700002 | * |
dc.identifier.scopusid | 2-s2.0-0344110140 | * |
dc.author.google | Yi H.C. | * |
dc.author.google | Joo S. | * |
dc.author.google | Nam K.H. | * |
dc.author.google | Lee J.S. | * |
dc.author.google | Kang B.G. | * |
dc.author.google | Kim W.T. | * |
dc.contributor.scopusid | 이준승(7601452195) | * |
dc.date.modifydate | 20240423081003 | * |