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Antiviral effects of 28-deacetylsendanin on herpes simplex virus-1 replication

Title
Antiviral effects of 28-deacetylsendanin on herpes simplex virus-1 replication
Authors
Kim M.Kim S.K.Park B.N.Lee K.H.Min G.H.Seoh J.Y.Park C.G.Hwang E.S.Cha C.Y.Kook Y.H.
Ewha Authors
서주영
SCOPUS Author ID
서주영scopus
Issue Date
1999
Journal Title
Antiviral Research
ISSN
0166-3542JCR Link
Citation
vol. 43, no. 2, pp. 103 - 112
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The compound purified from the fruit of Melia azedarach exerted an antiviral effect on herpes simplex virus-1 (HSV-1) in Vero cells. It was identified as 28-deacetylsendanin (28-DAS). The 50% inhibitory concentration (IC50) of 28-DAS was 1.46 μg/ml without cytotoxicity at 400 μg/ml on Vero cells. Electron microscopy showed that low electron-dense cores of newly synthesized nucleocapsids remained in swollen nuclei and no extracellular virus particles were observed at 15 h p.i. Consistent with this result, it was confirmed by a plaque assay that few infectious progeny viruses were released from the 28-DAS-treated virus-infected cells at 24 h p.i. Intracellular viruses in 28-DAS-treated virus-infected cells were 23% of untreated and infected cells. The synthesis of thymidine kinase (TK) was reduced by 28-DAS at early stage. In conclusion, 28-DAS inhibited the replication of HSV-1, reduced the synthesis of HSV-1 TK, and led to the formation of defective nucleocapsids. Copyright (C) 1999 Elsevier Science B.V.
DOI
10.1016/S0166-3542(99)00037-6
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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