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Myeloid differentiation of human cord blood CD34+ cells during ex vivo expansion using thrombopoietin, flt3-ligand and/or granulocyte-colony stimulating factor

Title
Myeloid differentiation of human cord blood CD34+ cells during ex vivo expansion using thrombopoietin, flt3-ligand and/or granulocyte-colony stimulating factor
Authors
Yoo E.-S.Ryu K.-H.Park H.-Y.Seong C.-M.Chung W.-S.Kim S.-C.Choi Y.-M.Hahn M.-J.Woo S.-Y.Seoh J.-Y.
Ewha Authors
박혜영서주영김승철정화순유경하성주명유은선우소연
SCOPUS Author ID
박혜영scopus; 서주영scopus; 김승철scopus; 정화순scopus; 유경하scopus; 성주명scopus; 유은선scopus; 우소연scopus
Issue Date
1999
Journal Title
British Journal of Haematology
ISSN
0007-1048JCR Link
Citation
vol. 105, no. 4, pp. 1034 - 1040
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
We investigated the phenotypic changes of human umbilical cord blood (CB) CD34+ cells during ex vivo expansion using thrombopoietin (TPO), flt3- ligand (FL), and/or granulocyte-colony stimulating factor (G-CSF). During ex vivo expansion of CD34+ cells isolated from human CB for up to 5 weeks, surface expression of molecules on the cultured cells including CD64 (FcγRI), CD32 (FcγRII). CD16 (FcγRIII), CD11b (MAC-1) and CD18 (β2- integrin) was analysed by flow cytometry along with simultaneous measurement of apoptosis by 7-aminoactinomycin D staining method. CD64, CD32 and/or CD18 expressing cells appeared in the cultures both with and without the addition of G-CSF until file tenth day. However, without G-CSF, CD16+ fractions did not appear and CD11b+ fractions were not maintained. With G-CSF, the CD16+ or CD11b+ fractions appeared only from the second week. These results suggest that G-CSF is necessary for the late stage of myeloid maturation during which CD16 and CD11b are expressed.
DOI
10.1046/j.1365-2141.1999.01456.x
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의학전문대학원 > 의학과 > Journal papers
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