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Myeloid differentiation of human cord blood CD34+ cells during ex vivo expansion using thrombopoietin, flt3-ligand and/or granulocyte-colony stimulating factor
- Title
- Myeloid differentiation of human cord blood CD34+ cells during ex vivo expansion using thrombopoietin, flt3-ligand and/or granulocyte-colony stimulating factor
- Authors
- Yoo E.-S.; Ryu K.-H.; Park H.-Y.; Seong C.-M.; Chung W.-S.; Kim S.-C.; Choi Y.-M.; Hahn M.-J.; Woo S.-Y.; Seoh J.-Y.
- Ewha Authors
- 박혜영; 서주영; 김승철; 정화순; 유경하; 성주명; 유은선; 우소연
- SCOPUS Author ID
- 박혜영; 서주영; 김승철; 정화순; 유경하; 성주명; 유은선; 우소연
- Issue Date
- 1999
- Journal Title
- British Journal of Haematology
- ISSN
- 0007-1048
- Citation
- British Journal of Haematology vol. 105, no. 4, pp. 1034 - 1040
- Indexed
- SCI; SCIE; SCOPUS
- Document Type
- Article
- Abstract
- We investigated the phenotypic changes of human umbilical cord blood (CB) CD34+ cells during ex vivo expansion using thrombopoietin (TPO), flt3- ligand (FL), and/or granulocyte-colony stimulating factor (G-CSF). During ex vivo expansion of CD34+ cells isolated from human CB for up to 5 weeks, surface expression of molecules on the cultured cells including CD64 (FcγRI), CD32 (FcγRII). CD16 (FcγRIII), CD11b (MAC-1) and CD18 (β2- integrin) was analysed by flow cytometry along with simultaneous measurement of apoptosis by 7-aminoactinomycin D staining method. CD64, CD32 and/or CD18 expressing cells appeared in the cultures both with and without the addition of G-CSF until file tenth day. However, without G-CSF, CD16+ fractions did not appear and CD11b+ fractions were not maintained. With G-CSF, the CD16+ or CD11b+ fractions appeared only from the second week. These results suggest that G-CSF is necessary for the late stage of myeloid maturation during which CD16 and CD11b are expressed.
- DOI
- 10.1046/j.1365-2141.1999.01456.x
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
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