Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박혜영 | * |
dc.contributor.author | 서주영 | * |
dc.contributor.author | 김승철 | * |
dc.contributor.author | 정화순 | * |
dc.contributor.author | 유경하 | * |
dc.contributor.author | 성주명 | * |
dc.contributor.author | 유은선 | * |
dc.contributor.author | 우소연 | * |
dc.date.accessioned | 2016-08-28T11:08:15Z | - |
dc.date.available | 2016-08-28T11:08:15Z | - |
dc.date.issued | 1999 | * |
dc.identifier.issn | 0007-1048 | * |
dc.identifier.other | OAK-242 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/218527 | - |
dc.description.abstract | We investigated the phenotypic changes of human umbilical cord blood (CB) CD34+ cells during ex vivo expansion using thrombopoietin (TPO), flt3- ligand (FL), and/or granulocyte-colony stimulating factor (G-CSF). During ex vivo expansion of CD34+ cells isolated from human CB for up to 5 weeks, surface expression of molecules on the cultured cells including CD64 (FcγRI), CD32 (FcγRII). CD16 (FcγRIII), CD11b (MAC-1) and CD18 (β2- integrin) was analysed by flow cytometry along with simultaneous measurement of apoptosis by 7-aminoactinomycin D staining method. CD64, CD32 and/or CD18 expressing cells appeared in the cultures both with and without the addition of G-CSF until file tenth day. However, without G-CSF, CD16+ fractions did not appear and CD11b+ fractions were not maintained. With G-CSF, the CD16+ or CD11b+ fractions appeared only from the second week. These results suggest that G-CSF is necessary for the late stage of myeloid maturation during which CD16 and CD11b are expressed. | * |
dc.language | English | * |
dc.title | Myeloid differentiation of human cord blood CD34+ cells during ex vivo expansion using thrombopoietin, flt3-ligand and/or granulocyte-colony stimulating factor | * |
dc.type | Article | * |
dc.relation.issue | 4 | * |
dc.relation.volume | 105 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1034 | * |
dc.relation.lastpage | 1040 | * |
dc.relation.journaltitle | British Journal of Haematology | * |
dc.identifier.doi | 10.1046/j.1365-2141.1999.01456.x | * |
dc.identifier.wosid | WOS:000081262800026 | * |
dc.identifier.scopusid | 2-s2.0-0032990429 | * |
dc.author.google | Yoo E.-S. | * |
dc.author.google | Ryu K.-H. | * |
dc.author.google | Park H.-Y. | * |
dc.author.google | Seong C.-M. | * |
dc.author.google | Chung W.-S. | * |
dc.author.google | Kim S.-C. | * |
dc.author.google | Choi Y.-M. | * |
dc.author.google | Hahn M.-J. | * |
dc.author.google | Woo S.-Y. | * |
dc.author.google | Seoh J.-Y. | * |
dc.contributor.scopusid | 박혜영(7601567979) | * |
dc.contributor.scopusid | 서주영(6603709174;57209001625) | * |
dc.contributor.scopusid | 김승철(35264000100) | * |
dc.contributor.scopusid | 정화순(7401983201;54885771500) | * |
dc.contributor.scopusid | 유경하(14038236200) | * |
dc.contributor.scopusid | 성주명(7005537065) | * |
dc.contributor.scopusid | 유은선(20936704200) | * |
dc.contributor.scopusid | 우소연(7402853365) | * |
dc.date.modifydate | 20240301081003 | * |