Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김화정 | * |
dc.date.accessioned | 2016-08-28T11:08:10Z | - |
dc.date.available | 2016-08-28T11:08:10Z | - |
dc.date.issued | 1999 | * |
dc.identifier.issn | 1225-8687 | * |
dc.identifier.other | OAK-151 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/218474 | - |
dc.description.abstract | Matrix metalloproteinase-2 (MMP-2; 72-kDa gelatinase; 72-kDa type IV collagenase; gelatinase A) plays an important role in normal physiological processes and in many pathologic processes such as arthritis and metastasis of cancer. Tissue inhibitor of metalloproteinases-2 (TIMP-2) binds to proMMP-2 or mature MMP-2 at a 1:1 ratio and inhibits the catalytic activity of MMP-2. We demonstrated that the baculovirus/ insect cell system does not have TIMP-2 activity. The human proMMP-2 free of TIMP-2 was expressed in the expression system and purified by one-step affinity chromatography using gelatin-Sepharose. The free proMMP-2 was autoactivated to the mature MMP-2 and autodegraded into smaller molecular weight forms in the absence of external activator. The activation and autodegradation of the proMMP-2 was much more rapid in the presence of 4-aminophenylmercuric acetate (APMA). Addition of TIMP-2 inhibits both APMA-induced activation and autodegradation of the free proMMP-2. However, an increasing concentration of TIMP-2 more readily inhibited activation of the free proMMP-2 than autodegradation. These results demonstrate that TIMP-2 plays roles in inhibition of both activation and autodegradation of the free proMMP-2 in addition to inhibition of the catalytic activity of MMP-2. | * |
dc.language | English | * |
dc.title | Tissue Inhibitor of Metalloproteinases-2 Inhibits the 4-Aminophenylmercuric Acetate-Induced Activation and Autodegradation of the Free Promatrix Metalloproteinase-2 | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 32 | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 60 | * |
dc.relation.lastpage | 66 | * |
dc.relation.journaltitle | Journal of Biochemistry and Molecular Biology | * |
dc.identifier.wosid | WOS:000078248000011 | * |
dc.identifier.scopusid | 2-s2.0-0033484579 | * |
dc.author.google | Jo Y. | * |
dc.author.google | Yoon D.-W. | * |
dc.author.google | Kim M.-Y. | * |
dc.author.google | Lee Y.-J. | * |
dc.author.google | Kim H.-J. | * |
dc.author.google | Lee S.-T. | * |
dc.contributor.scopusid | 김화정(56670336100) | * |
dc.date.modifydate | 20240118124308 | * |