Application of micellar electrokinetic capillary chromatography for the determination of benzoic acid and its esters in liquid formula medicines as preservatives

Abstract

We described a method for the simultaneous determination of preservatives including benzoic acid, methyl-, ethyl- and propyl-benzoate by micellar electrokinetic capillary chromatography (MECC). The factors affecting the reproducibility in the quantitative analysis of pharmaceuticals by MECC were investigated by varying the running buffer and washing condition inbetween runs. Preservatives in liquid formula medicines have been determined by optimum MECC condition using p-hydroxy benzoic acid as an internal standard. The reproducibility of this method was acceptable as a validate method for the quality control of pharmaceuticals (RSD<2%). Routine quantitative analysis of pharmaceuticals using MECC could be possible with well characterized reproducible procedure. Copyright (C) 1998 Elsevier Science B.V.