View : 149 Download: 0

Interaction between Norfloxacin and Single Stranded DNA

Title
Interaction between Norfloxacin and Single Stranded DNA
Authors
Yeo J.-A.Cho T.-S.Kim S.K.Moon H.R.Jhon G.-J.Nam W.
Ewha Authors
전길자남원우
SCOPUS Author ID
전길자scopus; 남원우scopus
Issue Date
1998
Journal Title
Bulletin of the Korean Chemical Society
ISSN
0253-2964JCR Link
Citation
vol. 19, no. 4, pp. 449 - 457
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
We compared various spectroscopic properties of a norfloxacin-single stranded DNA complex with those of norfloxacin-double stranded DNA complex. Norfloxacin binds to both double- and single stranded DNA, and we observed the following spectroscopic changes for both complexes: hypochromism in the norfloxacin absorption region in the absorption spectrum, the characteristic induced CD spectrum consisting of a weak positive band at 323 nm and a strong positive band at 280-300 nm followed by a negative band in the 260 nm region, a strong decrease in the fluorescence intensity and a red-shift in the fluorescence emission spectrum, and shorter fluorescence decay times. These results indicate that the environments of the bound norfloxacin in both DNAs are similar, although the equilibrium constant of the norfloxacin-single stranded DNA was twice as high as the norfloxacin-double stranded DNA complex. Both complexes were thermodynamically favored with similar negative ΔGo. Negative ΔHo terms contribute to these spontaneous reactions; ΔSo term was unfavorable.
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE