Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 박진병 | * |
dc.date.accessioned | 2016-08-28T11:08:01Z | - |
dc.date.available | 2016-08-28T11:08:01Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 2045-2322 | * |
dc.identifier.other | OAK-19082 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/218376 | - |
dc.description.abstract | 3'-Untranslated region (3'UTR) engineering was investigated to improve solubility of heterologous proteins (e.g., Baeyer-Villiger monooxygenases (BVMOs)) in Escherichia coli. Insertion of gene fragments containing putative RNase E recognition sites into the 3'UTR of the BVMO genes led to the reduction of mRNA levels in E. coli. Importantly, the amounts of soluble BVMOs were remarkably enhanced resulting in a proportional increase of in vivo catalytic activities. Notably, this increase in biocatalytic activity correlated to the number of putative RNase E endonucleolytic cleavage sites in the 3'UTR. For instance, the biotransformation activity of the BVMO BmoF1 (from Pseudomonas fluorescens DSM50106) in E. coli was linear to the number of RNase E cleavage sites in the 3'UTR. In summary, 3'UTR engineering can be used to improve the soluble expression of heterologous enzymes, thereby fine-tuning the enzyme activity in microbial cells. | * |
dc.language | English | * |
dc.publisher | NATURE PUBLISHING GROUP | * |
dc.title | 3 '-UTR engineering to improve soluble expression and fine-tuning of activity of cascade enzymes in Escherichia coli | * |
dc.type | Article | * |
dc.relation.volume | 6 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | SCIENTIFIC REPORTS | * |
dc.identifier.doi | 10.1038/srep29406 | * |
dc.identifier.wosid | WOS:000379388400001 | * |
dc.identifier.scopusid | 2-s2.0-84978884579 | * |
dc.author.google | Song, Ji-Won | * |
dc.author.google | Woo, Ji-Min | * |
dc.author.google | Jung, Gyoo Yeol | * |
dc.author.google | Bornscheuer, Uwe T. | * |
dc.author.google | Park, Jin-Byung | * |
dc.contributor.scopusid | 박진병(15036390700) | * |
dc.date.modifydate | 20240322114808 | * |