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dc.contributor.author남상집-
dc.date.accessioned2017-08-28T08:35:39Z-
dc.date.available2017-08-28T08:35:39Z-
dc.date.issued2016-
dc.identifier.issn0253-6269-
dc.identifier.issn1976-3786-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/218356-
dc.description.abstractbeta-N-acetylglucosamine (beta-AG) is a monosaccharide distributed widely in living organisms with various pivotal roles. The presence of particulates and impurities can affect the safety and efficacy of a product for its intended duration of use. Thus, the current study was carried out to identify and quantify the potentially-harmful process related impurities; namely alpha-N,6-diacetylglucosamine (alpha-DAG) and alpha-N-acetylglucosamine (alpha-AG), derived from the chemical and enzymatic synthesis of beta-AG. The impurities were characterized using a high resolution mass spectrometry, a nuclear magnetic resonance spectroscopy, and liquid chromatography-tandem mass spectrometry (LC/MS/MS). The developed method showed a good linearity (R (2) a parts per thousand yen 0.998), satisfactory precision (a parts per thousand currency sign6.1 % relative standard deviation), intra- and inter-day accuracy (88.20-97.50 %), extraction recovery (89.30-110.50 %), matrix effect (89.70-105.20 %), and stability (92.70-101.60 %). The method was successfully applied to determine the level of alpha-DAG that was 3.04 and 0.07 % of the total beta-AG, following chemical and enzymatic methods, respectively. It can be concluded that the enzymatic rather than the chemical method is more efficient for the synthesis of beta-AG. Characterization of impurities heeds the signal for acquiring and evaluating data that establishes biological safety.-
dc.languageEnglish-
dc.publisherPHARMACEUTICAL SOC KOREA-
dc.subjectbeta-N-acetylglucosamine-
dc.subjectProcess-related impurity-
dc.subjectTandem mass spectrometry-
dc.subjectChitin-
dc.subjectNuclear magnetic resonance-
dc.titleDetermination of process-related impurities in N-acetylglucosamine prepared by chemical and enzymatic methods: structural elucidation and quantification-
dc.typeArticle-
dc.relation.issue7-
dc.relation.volume39-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.indexKCI-
dc.relation.startpage937-
dc.relation.lastpage945-
dc.relation.journaltitleARCHIVES OF PHARMACAL RESEARCH-
dc.identifier.doi10.1007/s12272-016-0755-3-
dc.identifier.wosidWOS:000379692900007-
dc.identifier.scopusid2-s2.0-84976606339-
dc.author.googleKim, Yi Soo-
dc.author.googleLee, Sung Joong-
dc.author.googleChoi, Jin Young-
dc.author.googleKim, Yun-Hi-
dc.author.googleDesta, Kebede Taye-
dc.author.googlePiao, Zhe-
dc.author.googleChoi, Su-Lim-
dc.author.googleNam, Sang-Jip-
dc.author.googleKang, Kyung-Yun-
dc.author.googleAbd El-Aty, A. M.-
dc.author.googleShin, Yong Chul-
dc.author.googleShin, Sung Chul-
dc.contributor.scopusid남상집(36455982300)-
dc.date.modifydate20170601144635-
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자연과학대학 > 화학·나노과학전공 > Journal papers
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