Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 남상집 | * |
dc.date.accessioned | 2016-08-28T11:08:58Z | - |
dc.date.available | 2016-08-28T11:08:58Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 0253-6269 | * |
dc.identifier.issn | 1976-3786 | * |
dc.identifier.other | OAK-18993 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/218356 | - |
dc.description.abstract | beta-N-acetylglucosamine (beta-AG) is a monosaccharide distributed widely in living organisms with various pivotal roles. The presence of particulates and impurities can affect the safety and efficacy of a product for its intended duration of use. Thus, the current study was carried out to identify and quantify the potentially-harmful process related impurities; namely alpha-N,6-diacetylglucosamine (alpha-DAG) and alpha-N-acetylglucosamine (alpha-AG), derived from the chemical and enzymatic synthesis of beta-AG. The impurities were characterized using a high resolution mass spectrometry, a nuclear magnetic resonance spectroscopy, and liquid chromatography-tandem mass spectrometry (LC/MS/MS). The developed method showed a good linearity (R (2) a parts per thousand yen 0.998), satisfactory precision (a parts per thousand currency sign6.1 % relative standard deviation), intra- and inter-day accuracy (88.20-97.50 %), extraction recovery (89.30-110.50 %), matrix effect (89.70-105.20 %), and stability (92.70-101.60 %). The method was successfully applied to determine the level of alpha-DAG that was 3.04 and 0.07 % of the total beta-AG, following chemical and enzymatic methods, respectively. It can be concluded that the enzymatic rather than the chemical method is more efficient for the synthesis of beta-AG. Characterization of impurities heeds the signal for acquiring and evaluating data that establishes biological safety. | * |
dc.language | English | * |
dc.publisher | PHARMACEUTICAL SOC KOREA | * |
dc.subject | beta-N-acetylglucosamine | * |
dc.subject | Process-related impurity | * |
dc.subject | Tandem mass spectrometry | * |
dc.subject | Chitin | * |
dc.subject | Nuclear magnetic resonance | * |
dc.title | Determination of process-related impurities in N-acetylglucosamine prepared by chemical and enzymatic methods: structural elucidation and quantification | * |
dc.type | Article | * |
dc.relation.issue | 7 | * |
dc.relation.volume | 39 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 937 | * |
dc.relation.lastpage | 945 | * |
dc.relation.journaltitle | ARCHIVES OF PHARMACAL RESEARCH | * |
dc.identifier.doi | 10.1007/s12272-016-0755-3 | * |
dc.identifier.wosid | WOS:000379692900007 | * |
dc.identifier.scopusid | 2-s2.0-84976606339 | * |
dc.author.google | Kim, Yi Soo | * |
dc.author.google | Lee, Sung Joong | * |
dc.author.google | Choi, Jin Young | * |
dc.author.google | Kim, Yun-Hi | * |
dc.author.google | Desta, Kebede Taye | * |
dc.author.google | Piao, Zhe | * |
dc.author.google | Choi, Su-Lim | * |
dc.author.google | Nam, Sang-Jip | * |
dc.author.google | Kang, Kyung-Yun | * |
dc.author.google | Abd El-Aty, A. M. | * |
dc.author.google | Shin, Yong Chul | * |
dc.author.google | Shin, Sung Chul | * |
dc.contributor.scopusid | 남상집(57208839798) | * |
dc.date.modifydate | 20240220120010 | * |