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The dynamic transcriptional and translational landscape of the model antibiotic producer Streptomyces coelicolor A3(2)

Title
The dynamic transcriptional and translational landscape of the model antibiotic producer Streptomyces coelicolor A3(2)
Authors
Jeong, YujinKim, Ji-NuKim, Min WooBucca, GiseldaCho, SuhyungYoon, Yeo JoonKim, Byung-GeeRoe, Jung-HyeKim, Sun ChangSmith, Colin P.Cho, Byung-Kwan
Ewha Authors
윤여준
SCOPUS Author ID
윤여준scopus
Issue Date
2016
Journal Title
NATURE COMMUNICATIONS
ISSN
2041-1723JCR Link
Citation
vol. 7
Publisher
NATURE PUBLISHING GROUP
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Individual Streptomyces species have the genetic potential to produce a diverse array of natural products of commercial, medical and veterinary interest. However, these products are often not detectable under laboratory culture conditions. To harness their full biosynthetic potential, it is important to develop a detailed understanding of the regulatory networks that orchestrate their metabolism. Here we integrate nucleotide resolution genome-scale measurements of the transcriptome and translatome of Streptomyces coelicolor, the model antibiotic-producing actinomycete. Our systematic study determines 3,570 transcription start sites and identifies 230 small RNAs and a considerable proportion (similar to 21%) of leaderless mRNAs; this enables deduction of genome-wide promoter architecture. Ribosome profiling reveals that the translation efficiency of secondary metabolic genes is negatively correlated with transcription and that several key antibiotic regulatory genes are translationally induced at transition growth phase. These findings might facilitate the design of new approaches to antibiotic discovery and development.
DOI
10.1038/ncomms11605
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자연과학대학 > 화학·나노과학전공 > Journal papers
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