Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 손아정 | * |
dc.date.accessioned | 2016-08-27T04:08:12Z | - |
dc.date.available | 2016-08-27T04:08:12Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 0956-5663 | * |
dc.identifier.issn | 1873-4235 | * |
dc.identifier.other | OAK-18598 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/218216 | - |
dc.description.abstract | We demonstrated the detection of airborne bacteria by a disposable bio-precipitator and NanoGene assay combination. The bio-precipitator employed micro corona discharge at 1960 V and at less than 35 mu A to simultaneously charge, capture and lyse the airborne bacteria. This was enabled by the use of a 15 mu L. liquid anode. Using a custom exposure setup, the target bacterium Bacillus subtilis in the atomization solution was rendered airborne. After exposure, the liquid anode in the bio-precipitator was subsequently measured for DNA concentration and analyzed with the NanoGene assay. As the bacterial concentration increased from 0.0104 to 42.6 g-DCW/L the released DNA concentration in the liquid anode increased from 2.10 +/- 1.57 to 75.00 +/- 7.15 ng/mu L. More importantly, the NanoGene assay showed an increase in normalized fluorescence (gene quantification) from 18.03 +/- 1.18 to 49.71 +/- 1.82 as the bacterial concentrations increased from 0.0104 to 42.6 g-DCW/L. the electrical power consumption of the bioprecipitator was shown to be amenable for portable use. In addition, the detection limit of bio-precipitator and NanoGene assay combination in the context of environmentally relevant levels of airborne bacteria was also discussed. (C) 2016 Elsevier B.V. All rights reserved. | * |
dc.language | English | * |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | * |
dc.subject | Bio-precipitator | * |
dc.subject | Airborne bacteria | * |
dc.subject | Micro corona discharge | * |
dc.subject | Bacillus subtilis | * |
dc.subject | NanoGene assay | * |
dc.title | Detection of airborne bacteria with disposable bio-precipitator and NanoGene assay | * |
dc.type | Article | * |
dc.relation.volume | 83 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 205 | * |
dc.relation.lastpage | 212 | * |
dc.relation.journaltitle | BIOSENSORS & BIOELECTRONICS | * |
dc.identifier.doi | 10.1016/j.bios.2016.04.051 | * |
dc.identifier.wosid | WOS:000376802300029 | * |
dc.identifier.scopusid | 2-s2.0-84964545112 | * |
dc.author.google | Lee, Eun-Hee | * |
dc.author.google | Chua, Beelee | * |
dc.author.google | Son, Ahjeong | * |
dc.contributor.scopusid | 손아정(23487252600) | * |
dc.date.modifydate | 20240322131724 | * |