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Portable lysis apparatus for rapid single-step DNA extraction of Bacillus subtilis

Title
Portable lysis apparatus for rapid single-step DNA extraction of Bacillus subtilis
Authors
Lim, H. J.Lee, E. -H.Yoon, Y.Chua, B.Son, A.
Ewha Authors
손아정
SCOPUS Author ID
손아정scopus
Issue Date
2016
Journal Title
JOURNAL OF APPLIED MICROBIOLOGY
ISSN
1364-5072JCR Link1365-2672JCR Link
Citation
vol. 120, no. 2, pp. 379 - 387
Keywords
Bacillus subtilisbacterial DNA extractionlow costportable lysis apparatusrapid
Publisher
WILEY-BLACKWELL
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
AimsTo demonstrate and characterize a portable lysis apparatus for rapid single-step bacterial DNA extraction. Methods and ResultsOur portable lysis apparatus employed a novel design consisting of an annular piezo-element with perforated diaphragm. Using Bacillus subtilis as target bacteria, our portable lysis apparatus was able to achieve a normalized percent lysis as high as 66% within 30s. This is comparable to that by microprobe ultrasonication and almost 7 times higher than that by conventional bead beating. The effect from adding glass beads was predictable. However, the results from the addition of sodium dodecyl sulphate (SDS) were counter-intuitive because a further increase from 05 to 1% concentration reduced the lysis performance. The portable lysis apparatus is also at least 15-5 times more power efficient than microprobe ultrasonication. ConclusionsOur portable lysis apparatus is capable of rapidly extracting bacterial DNA and is more power efficient than microprobe ultrasonication. The addition of glass beads or SDS concentration (up to 05%) improves its performance. Significance and Impact of the StudyThe portable lysis apparatus provides a standalone, rapid, low cost and power efficient way of obtaining genomic constituents prior to a variety of bioassays used in the field of environmental, biomedical and other applied microbiology.
DOI
10.1111/jam.13011
Appears in Collections:
엘텍공과대학 > 환경공학전공 > Journal papers
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