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Cyclohexanone-induced stress metabolism of Escherichia coli and Corynebacterium glutamicum

Title
Cyclohexanone-induced stress metabolism of Escherichia coli and Corynebacterium glutamicum
Authors
Lee, Na-RaeYun, Ji-YeongLee, Sun-MeePark, Jin-Byung
Ewha Authors
박진병
SCOPUS Author ID
박진병scopus
Issue Date
2015
Journal Title
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
ISSN
1226-8372JCR Link1976-3816JCR Link
Citation
vol. 20, no. 6, pp. 1088 - 1098
Keywords
Escherichia coliCorynebacterium glutamicumcyclohexanonesolvent stressconstraints-based flux analysis
Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
Solvent stress occurs during whole-cell biocatalysis of organic chemicals. Organic substrates and/or products may accumulate in the cellular membranes of whole cells, causing structural destabilization of the membranes, which leads to disturbances in cellular carbon and energy metabolism. Here, we investigate the effect of cyclohexanone on carbon metabolism in Escherichia coli BL21 and Corynebacterium glutamicum ATCC13032. Adding cyclohexanone to the culture medium (i.e., glucose mineral medium) resulted in a decreased specific growth rate and increased cellular maintenance energy in both strains of bacteria. Notably, carbon metabolism, which is mainly involved to increase cellular maintenance energy, was very different between the bacteria. Carbon flux into the acetic acid fermentation pathway was dominantly enhanced in E. coli, whereas the TCA cycle appeared to be activated in C. glutamicum. In fact, carbon flux into the TCA cycle in E. coli appeared to be reduced with increasing amounts of cyclohexanone in the culture medium. Metabolic engineering of E. coli cells to maintain or improve TCA cycle activity and, presumably, that of the electron transport chain, which are involved in regeneration of cofactors (e.g., NAD(P)H and ATP) and formation of toxic metabolites (e.g., acetic acid), may be useful in increasing solvent tolerance and biotransformation of organic chemicals (e.g., cyclohexanone).
DOI
10.1007/s12257-015-0607-x
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
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