Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 배승진 | * |
dc.contributor.author | 임경민 | * |
dc.date.accessioned | 2016-08-27T04:08:36Z | - |
dc.date.available | 2016-08-27T04:08:36Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 1056-8719 | * |
dc.identifier.issn | 1873-488X | * |
dc.identifier.other | OAK-16086 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/217865 | - |
dc.description.abstract | In order for a novel test method to be applied for regulatory purposes, its reliability and relevance, i.e., reproducibility and predictive capacity, must be demonstrated. Here, we examine the predictive capacity of a novel non-radioisotopic local lymph node assay, LLNA: BrdU-FCM (5-bromo-2'-deoxyuridine-flow cytometry), with a cutoff approach and inferential statistics as a prediction model. 22 reference substances in OECD TG429 were tested with a concurrent positive control, hexylcinnamaldehyde 25%(PC), and the stimulation index (SI) representing the fold increase in lymph node cells over the vehicle control was obtained. The optimal cutoff SI (2.7 <= cutoff <3.5), with respect to predictive capacity, was obtained by a receiver operating characteristic curve, which produced 90.9% accuracy for the 22 substances. To address the inter-test variability in responsiveness, SI values standardized with PC were employed to obtain the optimal percentage cutoff (42.6 <= cutoff <57.3% of PC), which produced 86.4% accuracy. A test substance may be diagnosed as a sensitizer if a statistically significant increase in SI is elicited. The parametric one-sided t-test and non-parametric Wilcoxon rank-sum test produced 77.3% accuracy. Similarly, a test substance could be defined as a sensitizer if the SI means of the vehicle control, and of the low, middle, and high concentrations were statistically significantly different, which was tested using ANOVA or Kruskal-Wallis, with post hoc analysis, Dunnett, or DSCF (Dwass-Steel-Critchlow-Fligner), respectively, depending on the equal variance test, producing 81.8% accuracy. The absolute SI-based cutoff approach produced the best predictive capacity, however the discordant decisions between prediction models need to be examined further. (C) 2015 Elsevier Inc. All rights reserved. | * |
dc.language | English | * |
dc.publisher | ELSEVIER SCIENCE INC | * |
dc.subject | Local lymph node assay | * |
dc.subject | LLNA: BrdU-FCM | * |
dc.subject | Prediction model | * |
dc.subject | Skin sensitization | * |
dc.subject | Predictive capacity | * |
dc.subject | Descriptive and inferential statistics | * |
dc.title | Predictive capacity of a non-radioisotopic local lymph node assay using flow cytometry, LLNA: BrdU-FCM: Comparison of a cutoff approach and inferential statistics | * |
dc.type | Article | * |
dc.relation.volume | 78 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 76 | * |
dc.relation.lastpage | 84 | * |
dc.relation.journaltitle | JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS | * |
dc.identifier.doi | 10.1016/j.vascn.2015.12.001 | * |
dc.identifier.wosid | WOS:000370878600008 | * |
dc.identifier.scopusid | 2-s2.0-84949780953 | * |
dc.author.google | Kim, Da-eun | * |
dc.author.google | Yang, Hyeri | * |
dc.author.google | Jang, Won-Hee | * |
dc.author.google | Jung, Kyoung-Mi | * |
dc.author.google | Park, Miyoung | * |
dc.author.google | Choi, Jin Kyu | * |
dc.author.google | Jung, Mi-Sook | * |
dc.author.google | Jeon, Eun-Young | * |
dc.author.google | Heo, Yong | * |
dc.author.google | Yeo, Kyung-Wook | * |
dc.author.google | Jo, Ji-Hoon | * |
dc.author.google | Park, Jung Eun | * |
dc.author.google | Sohn, Soo Jung | * |
dc.author.google | Kim, Tae Sung | * |
dc.author.google | Ahn, Il Young | * |
dc.author.google | Jeong, Tae-Cheon | * |
dc.author.google | Lim, Kyung-Min | * |
dc.author.google | Bae, SeungJin | * |
dc.contributor.scopusid | 배승진(12143102400) | * |
dc.contributor.scopusid | 임경민(8916551700) | * |
dc.date.modifydate | 20240527131426 | * |