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Quantitative Polymerase Chain Reaction for Microbial Growth Kinetics of Mixed Culture System

Title
Quantitative Polymerase Chain Reaction for Microbial Growth Kinetics of Mixed Culture System
Authors
Cotto, AdaLooper, Jessica K.Mota, Linda C.Son, Ahjeong
Ewha Authors
손아정
SCOPUS Author ID
손아정scopus
Issue Date
2015
Journal Title
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
ISSN
1017-7825JCR Link1738-8872JCR Link
Citation
vol. 25, no. 11, pp. 1928 - 1935
Keywords
Microbial growthMonodquantitative PCR (qPCR)Pseudomonas putidaEscherichia coli K12
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
Microbial growth kinetics is often used to optimize environmental processes owing to its relation to the breakdown of substrate (contaminants). However, the quantification of bacterial populations in the environment is difficult owing to the challenges of monitoring a specific bacterial population within a diverse microbial community. Conventional methods are unable to detect and quantify the growth of individual strains separately in the mixed culture reactor. This work describes a novel quantitative PCR (qPCR)-based genomic approach to quantify each species in mixed culture and interpret its growth kinetics in the mixed system. Batch experiments were performed for both single and dual cultures of Pseudomonas putida and Escherichia coli K12 to obtain Monod kinetic parameters (mu(max) and K-s). The growth curves and kinetics obtained by conventional methods (i.e., dry weight measurement and absorbance reading) were compared with that obtained by qPCR assay. We anticipate that the adoption of this qPCR-based genomic assay can contribute significantly to traditional microbial kinetics, modeling practice, and the operation of bioreactors, where handling of complex mixed cultures is required.
DOI
10.4014/jmb.1503.03090
Appears in Collections:
엘텍공과대학 > 환경공학전공 > Journal papers
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