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Inhibitory effect of brazilin on osteoclast differentiation and its mechanism of action

Title
Inhibitory effect of brazilin on osteoclast differentiation and its mechanism of action
Authors
Kim, JinheeLee, Hyo KeunChang, Tong-ShinKang, Ki SungHwang, Gwi Seo
Ewha Authors
창동신
SCOPUS Author ID
창동신scopus
Issue Date
2015
Journal Title
INTERNATIONAL IMMUNOPHARMACOLOGY
ISSN
1567-5769JCR Link1878-1705JCR Link
Citation
vol. 29, no. 2, pp. 628 - 634
Keywords
BrazilinCaesalpinia sappanOsteoclast differentiationBone loss
Publisher
ELSEVIER SCIENCE BV
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Brazilin isolated from Caesalpinia sappan has long been known as a natural red pigment Our study evaluated the inhibitory effect of brazilin on osteoclast differentiation and investigated its mechanism of action. Our results demonstrated that brazilin inhibited receptor activator of nuclear factor kappa-B ligand (RANKL)-mediated osteoclast differentiation in RAW264.7 cells in a dose-dependent manner, without any evidence of cytotoxicity. The mRNA expression of tartrate-resistant acid phosphatase (TRAP), nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1), matrix metalloproteinase 9 (MMP-9), and cathepsin K in RANKL-treated RAW264.7 cells was inhibited by brazilin treatment. Brazilin also decreased RANKL-induced expression of inflammatory mediator genes such as inducible nitric oxide synthase, iNOS; cydooxygenase (COX)-2, tumor necrosis factor (TNF)-alpha, and interleukin (IL)-6 and inhibited extracellular signal-regulated kinases (ERK) and nuclear factor kappa-light-chain-enhancer of activated B cell (NF-kappa B) p65 phosphorylation in RANKL-stimulated RAW264.7 cells. A lipopolysaccharide (LPS)-induced osteoporosis study was also performed to assess the effects of brazilin in vivo. Micro-computed tomography (CT) analysis of the femurs showed that LPS treatment causes bone loss in mice, but it was significantly attenuated after co-treatment with brazilin (100 mg/kg). Therefore, brazilin may have therapeutic potential in preventing bone loss. (C) 2015 Elsevier B.V. All rights reserved.
DOI
10.1016/j.intimp.2015.09.018
Appears in Collections:
약학대학 > 약학과 > Journal papers
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