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A disposable bacterial lysis cartridge (BLC) suitable for an in situ water-borne pathogen detection system

Title
A disposable bacterial lysis cartridge (BLC) suitable for an in situ water-borne pathogen detection system
Authors
Lee, Eun-HeeLim, Hyun JeongSon, AhjeongChua, Beelee
Ewha Authors
손아정이은희
SCOPUS Author ID
손아정scopus; 이은희scopusscopus
Issue Date
2015
Journal Title
ANALYST
ISSN
0003-2654JCR Link1364-5528JCR Link
Citation
vol. 140, no. 22, pp. 7776 - 7783
Publisher
ROYAL SOC CHEMISTRY
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
We constructed a disposable bacterial lysis cartridge (BLC) suitable for an in situ pathogen detection system. It had an in-built micro corona discharge based ozone generator that provided ozone for cell lysis. Using a custom sample handling platform, its performance was evaluated with a Gram-positive bacterium of Bacillus subtilis. It was capable of achieving a similar degree of lysis as a commercial ultrasonic dismembrator with a P-1 microprobe in 10 min at an air pump flow rate of 29.4 ml min(-1) and an ozone generator operating voltage of 1600 V. The lysing duration could be significantly reduced to 5 min by increasing the air pump flow rate and the ozone generator operating voltage as well as by the addition of sodium dodecyl sulfate (SDS).
DOI
10.1039/c5an01317h
Appears in Collections:
엘텍공과대학 > 환경공학전공 > Journal papers
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