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Antiproliferative and Apoptotic Activity of Chamaecyparis obtusa Leaf Extract against the HCT116 Human Colorectal Cancer Cell Line and Investigation of the Bioactive Compound by Gas Chromatography-Mass Spectrometry-Based Metabolomics

Title
Antiproliferative and Apoptotic Activity of Chamaecyparis obtusa Leaf Extract against the HCT116 Human Colorectal Cancer Cell Line and Investigation of the Bioactive Compound by Gas Chromatography-Mass Spectrometry-Based Metabolomics
Authors
Kim, Hye-YounLee, Seul-GiOh, Taek-JooLim, Sa RangKim, So-HyunLee, Hong JinKim, Young-SukChoi, Hyung-Kyoon
Ewha Authors
김영석
SCOPUS Author ID
김영석scopus
Issue Date
2015
Journal Title
MOLECULES
ISSN
1420-3049JCR Link
Citation
vol. 20, no. 10, pp. 18066 - 18082
Keywords
Chamaecyparis obtusahuman colorectal cancermetabolite profilinggas chromatography-mass spectrometryanthricin
Publisher
MDPI AG
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Chamaecyparis obtusa (CO) belongs to the Cupressaceae family, and it is found widely distributed in Japan and Korea. In this study, the anti-proliferative activities of the methanol and water extracts of CO leaves against a human colorectal cancer cell line (HCT116) were investigated. The methanol extract of CO leaves, at a concentration of 1.25 mu g/mL, exhibited anti-proliferative activity against HCT116 cells, while displaying no cytotoxicity against Chang liver cells. Comparative global metabolite profiling was performed using gas chromatography-mass spectrometry coupled with multivariate statistical analysis, and it was revealed that anthricin was the major compound contributing to the anti-proliferative activity. The activation of c-Jun N-terminal kinases played a key role in the apoptotic effect of the methanol extract of CO leaves in HCT116 human colon cancer cells. These results suggest that the methanol extract and anthricin derived from CO leaves might be useful in the development of medicines with anti-colorectal cancer activity.
DOI
10.3390/molecules201018066
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엘텍공과대학 > 식품공학전공 > Journal papers
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