Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 창동신 | * |
dc.contributor.author | 류재상 | * |
dc.date.accessioned | 2016-08-27T04:08:21Z | - |
dc.date.available | 2016-08-27T04:08:21Z | - |
dc.date.issued | 2015 | * |
dc.identifier.issn | 0891-5849 | * |
dc.identifier.issn | 1873-4596 | * |
dc.identifier.other | OAK-15838 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/217723 | - |
dc.description.abstract | Reactive oxygen species (ROS) produced upon collagen stimulation are implicated in propagating various platelet-activating pathways. Among ROS-producing enzymes, NADPH oxidase (NOX) is largely responsible for collagen receptor-dependent ROS production. Therefore, NOX has been proposed as a novel target for the development of antiplatelet agent. We here investigate whether resveratrol inhibits collagen-induced NOX activation and further examine the effects of resveratrol on ROS-dependent signaling pathways in collagen-stimulated platelets. Collagen-induced superoxide anion production in platelets was inhibited by resveratrol. Resveratrol suppressed collagen-induced phosphorylation of p47(Phox), a major regulatory subunit of NOX. Correlated with the inhibitory effects on NOX, resveratrol protected SH2 domain-containing protein tyrosine phosphatase-2 (SHP-2) from ROS-mediated inactivation and subsequently attenuated the specific tyrosine phosphorylation of key components (spleen tyrosine kinase, Vavl, Bruton's tyrosine kinase, and phospholipase C gamma 2) for collagen receptor signaling cascades. Resveratrol also inhibited downstream responses such as cytosolic calcium elevation, P-selectin surface exposure, and integrin-alpha Illa beta 3 activation. Furthermore, resveratrol inhibited platelet aggregation and adhesion in response to collagen. The antiplatelet effects of resveratrol through the inhibition of NOX-derived ROS production and subsequent oxidative inactivation of SHP-2 suggest that resveratrol is a potential compound for prevention and treatment of thrombovascular diseases. (C) 2015 Elsevier Inc. All rights reserved. | * |
dc.language | English | * |
dc.publisher | ELSEVIER SCIENCE INC | * |
dc.subject | Resveratrol | * |
dc.subject | Antiplatelet | * |
dc.subject | Reactive oxygen species | * |
dc.subject | NADPH oxidase | * |
dc.subject | SH-2 domain-containing protein tyrosine | * |
dc.subject | phosphatase | * |
dc.title | Resveratrol inhibits collagen-induced platelet stimulation through suppressing NADPH oxidase and oxidative inactivation of SH2 domain-containing protein tyrosine phosphatase-2 | * |
dc.type | Article | * |
dc.relation.volume | 89 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 842 | * |
dc.relation.lastpage | 851 | * |
dc.relation.journaltitle | FREE RADICAL BIOLOGY AND MEDICINE | * |
dc.identifier.doi | 10.1016/j.freeradbiomed.2015.10.413 | * |
dc.identifier.wosid | WOS:000366355800079 | * |
dc.identifier.scopusid | 2-s2.0-84944754599 | * |
dc.author.google | Jang, Ji Yong | * |
dc.author.google | Min, Ji Hyun | * |
dc.author.google | Wang, Su Bin | * |
dc.author.google | Chae, Yun Hee | * |
dc.author.google | Baek, Jin Young | * |
dc.author.google | Kim, Myunghee | * |
dc.author.google | Ryu, Jae-Sang | * |
dc.author.google | Chang, Tong-Shin | * |
dc.contributor.scopusid | 창동신(7404726037) | * |
dc.contributor.scopusid | 류재상(36081118200) | * |
dc.date.modifydate | 20231120165709 | * |