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Transplantation of human spleen into immunodeficient NOD/SCID IL2R gamma(null) mice generates humanized mice that improve functional B cell development

Title
Transplantation of human spleen into immunodeficient NOD/SCID IL2R gamma(null) mice generates humanized mice that improve functional B cell development
Authors
Chung, Yun ShinSon, Jin KyungChoi, BongkumPark, Jae BermChang, JunKim, Sung Joo
Ewha Authors
장준
SCOPUS Author ID
장준scopus
Issue Date
2015
Journal Title
CLINICAL IMMUNOLOGY
ISSN
1521-6616JCR Link1521-7035JCR Link
Citation
vol. 161, no. 2, pp. 308 - 315
Keywords
ThymusBoneSpleenCD34(+) cellsHumanized miceHematopoiesis
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
We previously generated humanized TB34N mice that received human fetal thymus (T), bone tissue (B) and fetal liver-derived (FL)-CD34(+) cells (34) in immunodeficient, NOD/SCID IL2R gamma(null) (N) mice. Although humanized TB34N mice had excellent hematopoiesis, here, we sought to further improve this model by additional transplantation of human spleen tissue (S) as a secondary hematopoietic tissue (TBS34N). The human spleen grafts were enlarged and differentiated into a similar morphology of adult humans, including follicular lymphoid structures with T- and B-cells. The TBS34N mice mimicked mature human immune system (HIS): mature T- and B-cells and follicular dendritic cells; activated germinal center B-cells expressing CD71, BR3(+) cells, memory B-cells and activation-induced cytidine deaminase(+) B-cells; CD138(+) plasma cells were enriched in the mouse spleen. HBsAg-specific hIgG antibodies were secreted into the sera of all TBS34N mice upon immunization with HBsAg. Taken together, the humanized TBS34N mice improved mature HIS and achieved adaptive antibody responses. (C) 2015 Elsevier Inc. All rights reserved.
DOI
10.1016/j.clim.2015.09.001
Appears in Collections:
약학대학 > 약학과 > Journal papers
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