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Cathepsin B Imaging to Predict Quality of Engineered Cartilage

Title
Cathepsin B Imaging to Predict Quality of Engineered Cartilage
Authors
Yhee, Ji YoungKim, Yong-JinRyu, Ju HeeYoon, Hong YeolChang, HyeyounPark, Jae HyungLee, HyukjinJang, Hyon-SeokJeong, UnyongKim, KwangmeyungKang, Sun-Woong
Ewha Authors
이혁진
SCOPUS Author ID
이혁진scopus
Issue Date
2015
Journal Title
MACROMOLECULAR BIOSCIENCE
ISSN
1616-5187JCR Link1616-5195JCR Link
Citation
vol. 15, no. 9, pp. 1224 - 1232
Keywords
cartilage tissue engineeringcathepsin Bdiagnosismolecular imagingtransplantation
Publisher
WILEY-V C H VERLAG GMBH
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Cathepsin B (CB)-specific molecular imaging probe is applied to monitor the changes of CB expression in 3D cultured chondrocytes during the chondrogenesis. The probe is synthesized with a CB-cleavable peptide linked to a near infrared fluorescence (NIRF) dye and a dark quencher, which can recover the NIRF signal in the presence of CB enzyme. The CB activities in two different sets of chondrocytes are comparatively analyzed. The chondrocytes with higher CB activity show more extensive area of chondrogenesis that CB molecular imaging directly reflects the chondrogenic potency of the cells. The CB probe is expected to provide a reliable prediction for the quality of engineered cartilage by visualizing the activity of the relevant enzyme in chondrocytes.
DOI
10.1002/mabi.201500215
Appears in Collections:
약학대학 > 약학과 > Journal papers
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