Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 배윤수 | * |
dc.date.accessioned | 2016-08-27T04:08:01Z | - |
dc.date.available | 2016-08-27T04:08:01Z | - |
dc.date.issued | 2015 | * |
dc.identifier.issn | 0906-6705 | * |
dc.identifier.issn | 1600-0625 | * |
dc.identifier.other | OAK-15521 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/217536 | - |
dc.description.abstract | House dust mites (HDMs) are known to trigger chronic inflammation through Toll-like receptors (TLRs) and their signalling cascades. In this study, we found that TLR2 ligation by HDMs induced the activation of dual oxidase 2 (Duox2) and nuclear factor-B (NF-B), leading to the production of pro-inflammatory cytokines in human keratinocytes. Stimulation of human keratinocytes with HDMs resulted in increases in interleukin-8 (IL-8) and chemokine (C-C motif) ligand 20 (CCL20) levels. However, pro-inflammatory cytokine production was abolished in keratinocytes transfected with TLR2 siRNA, indicating that HDM-induced cytokine production was mediated via TLR2 signalling. We also examined the function of Duox1/2 isozymes, which are primarily expressed in keratinocytes, in HDM-mediated pro-inflammatory cytokine production. Human keratinocytes transfected with control siRNA or Duox1 siRNA showed no inhibition of IL-8 or CCL20 production in response to HDMs, whereas the silencing of Duox2 expression resulted in a failure to induce cytokine production. Moreover, the phosphorylation and nuclear localization of RelA/p65, a component of NF-B, were induced by HDMs in human keratinocytes. Transfection of human keratinocytes with TLR2 siRNA or Duox2 siRNA resulted in the complete abolishment of RelA/p65 nuclear localization in response to HDMs. Taken together, these results indicate that the HDM-dependent TLR2-Duox2 signalling axis indeed promotes NF-B activation, which induces IL-8 and CCL20 production and mediates epidermal keratinocyte inflammation. | * |
dc.language | English | * |
dc.publisher | WILEY-BLACKWELL | * |
dc.subject | dual oxidase 2 | * |
dc.subject | house dust mite | * |
dc.subject | nuclear factor-B | * |
dc.subject | reactive oxygen species | * |
dc.subject | Toll-like receptors | * |
dc.title | Dual oxidase 2 is essential for house dust mite-induced pro-inflammatory cytokine production in human keratinocytes | * |
dc.type | Article | * |
dc.relation.issue | 12 | * |
dc.relation.volume | 24 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 936 | * |
dc.relation.lastpage | 941 | * |
dc.relation.journaltitle | EXPERIMENTAL DERMATOLOGY | * |
dc.identifier.doi | 10.1111/exd.12808 | * |
dc.identifier.wosid | WOS:000367480100009 | * |
dc.identifier.scopusid | 2-s2.0-84939833265 | * |
dc.author.google | Ko, Eunbi | * |
dc.author.google | Choi, Hyun | * |
dc.author.google | Park, Kkot-Nara | * |
dc.author.google | Park, Ju-Yearl | * |
dc.author.google | Lee, Tae Ryong | * |
dc.author.google | Shin, Dong Wook | * |
dc.author.google | Bae, Yun Soo | * |
dc.contributor.scopusid | 배윤수(15031067200) | * |
dc.date.modifydate | 20240415133331 | * |