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Oxidative DNA cleavage by Cu(II) complexes: Effect of periphery substituent groups
- Oxidative DNA cleavage by Cu(II) complexes: Effect of periphery substituent groups
- Wang, Wei; Lee, Young Ae; Kim, Gyeongwon; Kim, Seog K.; Lee, Ga Ye; Kim, Jinheung; Kim, Youngmee; Park, Gyeong Jin; Kim, Cheal
- Ewha Authors
- 김영미; 김진흥
- SCOPUS Author ID
- 김영미; 김진흥
- Issue Date
- Journal Title
- JOURNAL OF INORGANIC BIOCHEMISTRY
- 0162-0134; 1873-3344
- vol. 153, pp. 143 - 149
- Cu complexes; DNA cleavage; Dipicolylamine; Linear dichroism
- ELSEVIER SCIENCE INC
- SCI; SCIE; SCOPUS
- A series of structurally-related [Cu(R-benzyl-dipicolylamine)(NO3)(2)] complexes, where R = methoxy- (1), methyl- (2), H- (3), fluoro- (4), and nitro-group (5), were synthesized, and their activity on DNA cleavage was investigated by linear dichroism (ID) and electrophoresis. The addition of a benzyl group to the dipicolylamine ligand of the [Cu(dipicolylamine)(NO3)(2)] complex (A), i.e., the [Cu(benzyl-dipicolylamine)(NO3)(2)] complex (3), caused significant enhancement in the efficiency of oxidative cleavage of both super-coiled (sc) and double stranded (ds) DNA, as evidenced by the electrophoresis pattern and faster decrease in the LD intensity at 260 nm. The efficiency in DNA cleavage was also altered with further modifications of the benzyl group by the introduction of various substituents at the para-position. The cleavage efficiency appeared to be the largest when the methyl group was attached. The order of efficiency in DNA cleavage was methyl > methoxy approximate to H > fluoro approximate to nitro group. When an electron-withdrawing group was introduced, the cleavage efficiency decreased remarkably. The reactive oxygen species involved in the cleavage process were the superoxide radical and singlet oxygen. A possible mechanism for this variation in the DNA cleavage efficiency was proposed. (C) 2015 Elsevier Inc All rights reserved.
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