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Pyruvate metabolism: A therapeutic opportunity in radiation-induced skin injury

Title
Pyruvate metabolism: A therapeutic opportunity in radiation-induced skin injury
Authors
Yoo, HyunKang, Jeong WookLee, Dong WonOh, Sang HoLee, Yun-SilLee, Eun-JungCho, Jaeho
Ewha Authors
이윤실
SCOPUS Author ID
이윤실scopus
Issue Date
2015
Journal Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN
0006-291XJCR Link1090-2104JCR Link
Citation
vol. 460, no. 3, pp. 504 - 510
Keywords
RadiationSkin injuryPyruvate dehydrogenase kinase
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Ionizing radiation is used to treat a range of cancers. Despite recent technological progress, radiation therapy can damage the skin at the administration site. The specific molecular mechanisms involved in this effect have not been fully characterized. In this study, the effects of pyruvate, on radiation-induced skin injury were investigated, including the role of the pyruvate dehydrogenase kinase 2 (PDK2) signaling pathway. Next generation sequencing (NGS) identified a wide range of gene expression differences between the control and irradiated mice, including reduced expression of PDK2. This was confirmed using Q-PCR. Cell culture studies demonstrated that PDK2 overexpression and a high cellular pyruvate concentration inhibited radiation-induced cytokine expression. Immunohistochemical studies demonstrated radiation-induced skin thickening and gene expression changes. Oral pyruvate treatment markedly downregulated radiation-induced changes in skin thickness and inflammatory cytokine expression. These findings indicated that regulation of the pyruvate rnetabolic pathway could provide an effective approach to the control of radiation-induced skin damage. (C) 2015 Elsevier Inc. All rights reserved.
DOI
10.1016/j.bbrc.2015.03.060
Appears in Collections:
약학대학 > 약학과 > Journal papers
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