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Inhibitory effects of sulfur compounds on methane oxidation by a methane-oxidizing consortium

Title
Inhibitory effects of sulfur compounds on methane oxidation by a methane-oxidizing consortium
Authors
Lee, Eun-HeeMoon, Kyung-EunKim, Tae GwanLee, Sang-DonCho, Kyung-Suk
Ewha Authors
조경숙이상돈김태관이은희
SCOPUS Author ID
조경숙scopus; 이상돈scopus; 이은희scopusscopus
Issue Date
2015
Journal Title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN
1389-1723JCR Link1347-4421JCR Link
Citation
vol. 120, no. 6, pp. 670 - 676
Keywords
MethaneParticulate methane monooxygenasesMethanethiolHydrogen sulfideInteraction
Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Kinetic and enzymatic inhibition experiments were performed to investigate the effects of methanethiol (MT) and hydrogen sulfide (H2S) on methane oxidation by a methane-oxidizing consortium. In the coexistence of MT and H2S, the oxidation of methane was delayed until MT and H2S were completely degraded. MT and H2S could be degraded, both with and without methane. The kinetic analysis revealed that the methane-oxidizing consortium showed a maximum methane oxidation rate (V-max) of 3.7 mmol g-dry cell weight (DCW)(-1) h(-1) and a saturation constant (K-m) of 184.1 mu M. MT and H2S show competitive inhibition on methane oxidation, with inhibition values (K-i) of 1504.8 and 359.8 mu M, respectively. MT was primary removed by particulate methane monooxygenases (pMMO) of the consortium, while H2S was degraded by the other microorganisms or enzymes in the consortium. DNA and mRNA transcript levels of the pmoA gene expressions were decreased to similar to 10(6) and 10(3) pmoA gene copy number g-DCW-1 after MT and H2S degradation, respectively; however, both the amount of the DNA and mRNA transcript recovered their initial levels of similar to 10(7) and 10(5) pmoA gene copy number g-DCW-1 after methane oxidation, respectively. The gene expression results indicate that the pmoA gene could be rapidly reproducible after methane oxidation. This study provides comprehensive information of kinetic interactions between methane and sulfur compounds. (c) 2015, The Society for Biotechnology, Japan. All rights reserved.
DOI
10.1016/j.jbiosc.2015.04.006
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엘텍공과대학 > 환경공학전공 > Journal papers
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