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Micro corona discharge based cell lysis method suitable for inhibitor resistant bacterial sensing systems

Title
Micro corona discharge based cell lysis method suitable for inhibitor resistant bacterial sensing systems
Authors
Lee, Eun-HeeChua, BeeleeSon, Ahjeong
Ewha Authors
손아정이은희
SCOPUS Author ID
손아정scopus; 이은희scopusscopus
Issue Date
2015
Journal Title
SENSORS AND ACTUATORS B-CHEMICAL
ISSN
0925-4005JCR Link
Citation
vol. 216, pp. 17 - 23
Keywords
Cell lysisCorona dischargeInhibitor resistantBacterial detection systemsOzone
Publisher
ELSEVIER SCIENCE SA
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
We demonstrated a bacterial cell lysis method suitable for inhibitor resistant bacterial detection systems where purification of extracted DNA is not necessary. The presented method potentially improves the field portability of such systems. It allows cell lysis and DNA extraction to be performed without the use of bead mill, sonication, thermal cycling, additional reagents or enzymes. Bacterial cell lysis is achieved in a single step by pumping ozone generated by a micro corona discharge into the bacterial sample. The results with Pseudomonas putida as the target bacteria showed that it was capable of achieving 98.5 +/- 0.2% lysis (normalized to 1 min of sonication at 10 W) after 10 min of treatment at a flow rate of 38 ml/min and an applied voltage of 2000 V. By increasing the treatment duration, flow rate and applied voltage, the normalized % lysis could be increased. In addition, continuous and pulsed treatments yield similar normalized % cell lysis. (C) 2015 Elsevier B.V. All rights reserved.
DOI
10.1016/j.snb.2015.04.030
Appears in Collections:
엘텍공과대학 > 환경공학전공 > Journal papers
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