Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 정병문 | * |
dc.contributor.author | 황성주 | * |
dc.date.accessioned | 2016-08-27T04:08:29Z | - |
dc.date.available | 2016-08-27T04:08:29Z | - |
dc.date.issued | 2015 | * |
dc.identifier.issn | 1616-301X | * |
dc.identifier.issn | 1616-3028 | * |
dc.identifier.other | OAK-14906 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/217204 | - |
dc.description.abstract | 2D/3D hybrid cell culture systems are constructed by increasing the temperature of the thermogelling poly(ethylene glycol)-poly(L-alanine) diblock copolymer (PEG-L-PA) aqueous solution in which tonsil tissue-derived mesenchymal stem cells and graphene oxide (GO) or reduced graphene oxide (rGO) are suspended, to 37 degrees C. The cells exhibit spherical cell morphologies in 2D/3D hybrid culture systems of GO/PEG-L-PA and rGO/PEG-L-PA by using the growth medium. The cell proliferations are 30%-50% higher in the rGO/PEG-L-PA hybrid system than in the GO/PEG-L-PA hybrid system. When chondrogenic culture media enriched with TGF-beta 3 is used in the 2D/3D hybrid systems, cells extensively aggregate, and the expression of chondrogenic biomarkers of SOX 9, COL II A1, COL II, and COL X significantly increases in the GO/PEG-L-PA 2D/3D hybrid system as compared with the PEG-L-PA 3D systems and rGO/PEG-L-PA 2D/3D hybrid system, suggesting that the GO/PEG-L-PA 2D/3D hybrid system can be an excellent candidate as a chondrogenic differentiation platform of the stem cell. This paper also suggests that a 2D/3D hybrid system prepared by incorporating 2D materials with various surface biofunctionalities in the in situ forming 3D hydrogel matrix can be a new cell culture system. | * |
dc.language | English | * |
dc.publisher | WILEY-V C H VERLAG GMBH | * |
dc.title | 2D and 3D Hybrid Systems for Enhancement of Chondrogenic Differentiation of Tonsil-Derived Mesenchymal Stem Cells | * |
dc.type | Article | * |
dc.relation.issue | 17 | * |
dc.relation.volume | 25 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 2573 | * |
dc.relation.lastpage | 2582 | * |
dc.relation.journaltitle | ADVANCED FUNCTIONAL MATERIALS | * |
dc.identifier.doi | 10.1002/adfm.201500299 | * |
dc.identifier.wosid | WOS:000354200000010 | * |
dc.identifier.scopusid | 2-s2.0-84928729909 | * |
dc.author.google | Park, Jinhye | * |
dc.author.google | Kim, In Young | * |
dc.author.google | Patel, Madhumita | * |
dc.author.google | Moon, Hyo Jung | * |
dc.author.google | Hwang, Seong-Ju | * |
dc.author.google | Jeong, Byeongmoon | * |
dc.contributor.scopusid | 정병문(7102237959) | * |
dc.contributor.scopusid | 황성주(7404626171) | * |
dc.date.modifydate | 20240118155902 | * |