View : 22 Download: 0

A New-Generation Fluorescent-Based Metal Sensor - iLOV Protein

Title
A New-Generation Fluorescent-Based Metal Sensor - iLOV Protein
Authors
Ravikumar, YuvarajNadarajan, Saravanan PrabhuLee, Chong-SoonRhee, Jin-KyuYun, Hyungdon
Ewha Authors
이진규
SCOPUS Author ID
이진규scopus
Issue Date
2015
Journal Title
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
ISSN
1017-7825JCR Link1738-8872JCR Link
Citation
vol. 25, no. 4, pp. 503 - 510
Keywords
iLOV proteincopperfluorescence quenchingmetal sensor
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
The iLOV protein belongs to a family of blue-light photoreceptor proteins containing a light-oxygen-voltage sensing domain with a noncovalently bound flavin mononucleotide (FMN) as its chromophore. Owing to advantages such as its small size, oxygen-independent nature, and pH stability, iLOV is an ideal candidate over other reporter fluorescent proteins such as GFP and DsRed. Here, for the first time, we describe the feasibility of applying LOV domain-based fluorescent iLOV as a metal sensor by measuring the fluorescence quenching of a protein with respect to the concentration of metal ions. In the present study, we demonstrated the inherent copper sensing property of the iLOV protein and identified the possible amino acids responsible for metal binding. The fluorescence quenching upon exposure to Cu2+ was highly sensitive and exhibited reversibility upon the addition of the metal chelator EDTA. The copper binding constant was found to be 4.72 +/- 0.84 mu M. In addition, Cu2+-bound iLOV showed high fluorescence quenching at near physiological pH. Further computational analysis yielded a better insight into understanding the possible amino acids responsible for Cu2+ binding with the iLOV protein.
DOI
10.4014/jmb.1409.09035
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE