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Peroxiredoxin 3 has a crucial role in the contractile function of skeletal muscle by regulating mitochondrial homeostasis

Title
Peroxiredoxin 3 has a crucial role in the contractile function of skeletal muscle by regulating mitochondrial homeostasis
Authors
Lee, Kwang-PyoShin, Yeo JinCho, Sung ChunLee, Seung-MinBahn, Young JaeKim, Ji YoungKwon, Eun-SooJeong, Do YeunPark, Sang ChulRhee, Sue GooWoo, Hyun AeKwon, Ki-Sun
Ewha Authors
우현애
SCOPUS Author ID
우현애scopus
Issue Date
2014
Journal Title
FREE RADICAL BIOLOGY AND MEDICINE
ISSN
0891-5849JCR Link1873-4596JCR Link
Citation
vol. 77, pp. 298 - 306
Keywords
Peroxiredoxin 3MitochondriaSkeletal muscleMyoblastMyogenesisMitofusinReactive oxygen speciesOxidative stressFatigueFree radicals
Publisher
ELSEVIER SCIENCE INC
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
Antioxidant systems against reactive oxygen species (ROS) are important factors in regulating homeostasis in various cells, tissues, and organs. Although ROS are known to cause to muscular disorders, the effects of mitochondrial ROS in muscle physiology have not been fully understood. Here, we investigated the effects of ROS on muscle mass and function using mice deficient in peroxiredoxin 3 (Prx3), which is a mitochondrial antioxidant protein. Ablation of Prx3 deregulated the mitochondrial network and membrane potential of myotubes, in which ROS levels were increased. We showed that the DNA content of mitochondria and ATP production were also reduced in Prx3-KO muscle. Of note, the mitofusin 1 and 2 protein levels decreased in Prx3-KO muscle, a biochemical evidence of impaired mitochondrial fusion. Contractile dysfunction was examined by measuring isometric forces of isolated extensor digitorum longus (EDL) and soleus muscles. Maximum absolute forces in both the EDL and the soleus muscles were not significantly affected in Prx3-KO mice. However, fatigue trials revealed that the decrease in relative force was greater and more rapid in soleus from Prx3-KO compared to wild-type mice. Taken together, these results suggest that Prx3 plays a crucial role in mitochondrial homeostasis and thereby controls the contractile functions of skeletal muscle. (C) 2014 Elsevier Inc. All rights reserved.
DOI
10.1016/j.freeradbiomed.2014.09.010
Appears in Collections:
약학대학 > 약학과 > Journal papers
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