Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이윤실 | * |
dc.date.accessioned | 2016-08-27T04:08:45Z | - |
dc.date.available | 2016-08-27T04:08:45Z | - |
dc.date.issued | 2013 | * |
dc.identifier.issn | 0021-9258 | * |
dc.identifier.other | OAK-10844 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/216794 | - |
dc.description.abstract | Background: The microRNA that regulates the expression of CAGE is unknown. Results: miR-200b and CAGE exert opposite regulations on the response to microtubule-targeting drugs, invasion, tumorigenic potential, and angiogenic potential. Conclusion: CAGE and miR-200b form a feedback regulatory loop. Significance: miR-200b may be a target for the treatment of CAGE-driven cancers. Cancer/testis antigen cancer-associated gene (CAGE) is known to be involved in various cellular processes, such as proliferation, cell motility, and anti-cancer drug resistance. However, the mechanism of the expression regulation of CAGE remains unknown. Target scan analysis predicted the binding of microRNA-200b (miR-200b) to CAGE promoter sequences. The expression of CAGE showed an inverse relationship with miR-200b in various cancer cell lines. miR-200b was shown to bind to the 3-UTR of CAGE and to regulate the expression of CAGE at the transcriptional level. miR-200b also enhanced the sensitivities to microtubule-targeting drugs in vitro. miR-200b and CAGE showed opposite regulations on invasion potential and responses to microtubule-targeting drugs. Xenograft experiments showed that miR-200b had negative effects on the tumorigenic and metastatic potential of cancer cells. The effect of miR-200b on metastatic potential involved the expression regulation of CAGE by miR-200b. miR-200b decreased the tumorigenic potential of a cancer cell line resistant to microtubule-targeting drugs in a manner associated with the down-regulation of CAGE. ChIP assays showed the direct regulation of miR-200b by CAGE. CAGE enhanced the invasion potential of a cancer cell line stably expressing miR-200b. miR-200b exerted a negative regulation on tumor-induced angiogenesis. The down-regulation of CAGE led to the decreased expression of plasminogen activator inhibitor-1, a TGF-responsive protein involved in angiogenesis, and VEGF. CAGE mediated tumor-induced angiogenesis and was necessary for VEGF-promoted angiogenesis. Human recombinant CAGE protein displayed angiogenic potential. Thus, miR-200b and CAGE form a feedback regulatory loop and regulate the response to microtubule-targeting drugs, as well as the invasion, tumorigenic potential, and angiogenic potential. | * |
dc.language | English | * |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | * |
dc.subject | Angiogenesis | * |
dc.subject | Anticancer Drug | * |
dc.subject | Cancer Biology | * |
dc.subject | Invasion | * |
dc.subject | MicroRNA | * |
dc.subject | Angiogenic Potential | * |
dc.subject | CAGE | * |
dc.subject | Feedback Regulatory Loop | * |
dc.subject | Tumorigenic Potential | * |
dc.subject | miR-200b | * |
dc.title | miR-200b and Cancer/Testis Antigen CAGE Form a Feedback Loop to Regulate the Invasion and Tumorigenic and Angiogenic Responses of a Cancer Cell Line to Microtubule-targeting Drugs | * |
dc.type | Article | * |
dc.relation.issue | 51 | * |
dc.relation.volume | 288 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 36502 | * |
dc.relation.lastpage | 36518 | * |
dc.relation.journaltitle | JOURNAL OF BIOLOGICAL CHEMISTRY | * |
dc.identifier.doi | 10.1074/jbc.M113.502047 | * |
dc.identifier.wosid | WOS:000328883400031 | * |
dc.author.google | Kim, Youngmi | * |
dc.author.google | Park, Deokbum | * |
dc.author.google | Kim, Hyuna | * |
dc.author.google | Choi, Munseon | * |
dc.author.google | Lee, Hansoo | * |
dc.author.google | Lee, Yun Sil | * |
dc.author.google | Choe, Jongseon | * |
dc.author.google | Kim, Young Myeong | * |
dc.author.google | Jeoung, Dooil | * |
dc.contributor.scopusid | 이윤실(17137192000) | * |
dc.date.modifydate | 20240130115944 | * |