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Intercalation of bulky Delta,Delta- and Lambda,Lambda-bis-Ru(II) complex between DNA base pairs

Title
Intercalation of bulky Delta,Delta- and Lambda,Lambda-bis-Ru(II) complex between DNA base pairs
Authors
Jang, Yoon JungKwon, Byung-HyangChoi, Byung-HoonBae, Chang HwanSeo, Mi SookNam, WonwooKim, Seog K.
Ewha Authors
남원우서미숙
SCOPUS Author ID
남원우scopus
Issue Date
2008
Journal Title
JOURNAL OF INORGANIC BIOCHEMISTRY
ISSN
0162-0134JCR Link
Citation
vol. 102, no. 10, pp. 1885 - 1891
Keywords
Ru(II) complexintercalationDNAcircular dichroismlinear dichroismmetal complex
Publisher
ELSEVIER SCIENCE INC
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
Interaction of Delta,Delta- and Lambda,Lambda-bis-Ru(II) complexes with native DNA was investigated by isotropic absorption and polarized spectroscopy including circular and linear dichroism (CD and LD). Despite the steric hindrance originating from its four bulky phenanthroline ligands at both ends of the molecule, this molecule rapidly intercalates between DNA base pairs. Intercalation was judged by large hypochromism and red shift in the UV-visible absorption spectra in the absorption region of the bridging moiety as well as in the metal-to-ligand-charge transfer absorption region. Further support for the intercalation is found in the fact that the magnitude of negative reduced LD signal in the absorption region of the bridging moiety was comparable to that of the DNA absorption region, indicating that the bridge connecting the two Ru(II) complexes is nearly parallel to the DNA base planes. No difference in the binding mode between the two enantiomers was observed. in the presence of either bis-Ru(II) complex, ethidium bromide, a classical intercalator, can intercalate into the empty sites but was not able to replace the Ru(II) complexes. Near the saturation, ground state interaction between ethidium and bis-Ru(II) complex was evident by LD. (c) 2008 Elsevier Inc. All rights reserved.
DOI
10.1016/j.jinorgbio.2008.06.012
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자연과학대학 > 화학·나노과학전공 > Journal papers
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