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Genetic toxicity test of o-Nitrotoluene by ames, micronucleus, comet assays and microarray analysis

Title
Genetic toxicity test of o-Nitrotoluene by ames, micronucleus, comet assays and microarray analysis
Authors
Lee, Eun MiLee, So YounLee, Woo SunKang, Jin SeokHan, EuisikGo, Seo YounSheen, Yhun YongKim, Seung HeePark, Sue Nie
Ewha Authors
신윤용
SCOPUS Author ID
신윤용scopus
Issue Date
2007
Journal Title
MOLECULAR & CELLULAR TOXICOLOGY
ISSN
1738-642XJCR Link
Citation
vol. 3, no. 2, pp. 107 - 112
Keywords
o-Nitrotolueneames testCOMETMN assaymicroarrayS9 fraction
Publisher
KOREAN SOC TOXICOGENOMICS & TOXICOPROTEOMICS
Indexed
SCIE; SCOPUS; KCI WOS
Abstract
o-Nitrotoluene is used to synthesize artificial dyes and raw materials of urethane resin. In this study, we have carried out in vitro genetic toxicity tests and microarray analysis to understand the underlying mechanisms and the mode of action of toxicity of o-nitrotoluene. TA1535 and TA98 cells were treated with o-nitrotoluene to test its toxicity by basic genetic toxicity test. Ames and two new in vitro micronucleus and COMET assays were applied using CHO cells and L5178Y cells, respectively. In addition, microarray analysis of differentially expressed genes in L5178Y cells in response to o-nitrotoluene was analyzed using Affymatrix genechip. The result of Ames test was that o-nitrotoluene treatment did not increase the mutations both in base substitution strain TA1535 and in frame shift TA98. o-Nitrotoluene has not increased micronuclei in CHO cells. But o-nitrotoluene increased DNA damage in L5178Y cell. Two-hundred two genes were initially selected as differentially expressed genes in response to o-nitrotoluene by microarray analysis and forty four genes among them were over 2 times of log fold changed. These forty four genes could be candidate biomarkers of genetic toxic action of o-nitrotoluene related to induction of mutation and/or induction of micronuclei and DNA damage. Further confirmation of these candidate markers related to the DNA damage will be useful to understand the detailed mechanism of action of o-nitrotoluene.
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약학대학 > 약학과 > Journal papers
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