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Ionising radiation induces changes associated with epithelial-mesenchymal transdifferentiation and increased cell motility of A549 lung epithelial cells

Title
Ionising radiation induces changes associated with epithelial-mesenchymal transdifferentiation and increased cell motility of A549 lung epithelial cells
Authors
Jung, Jae-WonHwang, So-YoungHwang, Ji-SunOh, Eok-SooPark, SeokheeHan, Inn-Oc
Ewha Authors
오억수
SCOPUS Author ID
오억수scopus
Issue Date
2007
Journal Title
EUROPEAN JOURNAL OF CANCER
ISSN
0959-8049JCR Link
Citation
vol. 43, no. 7, pp. 1214 - 1224
Keywords
radiationEMTmigration
Publisher
ELSEVIER SCI LTD
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
Radiotherapy remains a major therapeutic option for patients with advanced lung cancer. Nevertheless, the effects of irradiation on malignant biological behaviours (e.g. migration and transformation of cancer cells) have yet to be clarified. We conducted an in vitro study to investigate the radiation-induced alterations including morphology, adhesion, and cell motility of A549 human lung cancer cells. These changes, which are associated with epithelial-mesenchymal transdifferentiation (EMT), seem to be linked to radiation-induced fibrosis, which represents one of the most common long-term adverse effects of curative radiotherapy. In addition, loss of intercellular adhesion and increased cell motility may be involved in post-radiotherapy-associated metastasis. We showed that stress fibres and focal adhesions are increased and that cell-cell junctions are decreased in response to ionising radiation. Radiation also significantly increased cell motility. The p38-specific inhibitor, SB203580, reduced the radiation-promoted migration of A549 cells, whereas SP600125, a JNK MAPK-specific inhibitor, inhibited both inherent and radiation-mediated cell motility. Consistent with this observation, radiation up-regulated the phosphorylation of p38 MAPK. Current approaches to cancer treatment involving more intensive radiotherapy regimens have been suggested to be associated with a higher incidence of local or distant metastasis. Therefore, a subset of patients may benefit from a combination of radiotherapy with inhibitors of EMT or cell migration. (c) 2007 Elsevier Ltd. All rights reserved.
DOI
10.1016/j.ejca.2007.01.034
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자연과학대학 > 생명과학전공 > Journal papers
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