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Monitoring of microbial diversity and activity during bioremediation of crude OH-contaminated soil with different treatments

Title
Monitoring of microbial diversity and activity during bioremediation of crude OH-contaminated soil with different treatments
Authors
Baek, Kyung-HwaYoon, Byung-DaeKim, Byung-HyukCho, Dae-HyunLee, In-SookOh, Hee-MockKim, Hee-Sik
Ewha Authors
이인숙
SCOPUS Author ID
이인숙scopusscopus
Issue Date
2007
Journal Title
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
ISSN
1017-7825JCR Link
Citation
vol. 17, no. 1, pp. 67 - 73
Keywords
alkB genebioaugmentationbiostimulationbiosurfactantdenaturing gradient gel electrophoresismicrobial community
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
The present study compared the microbial diversity and activity during the application of various bioremediation processes to crude oil-contaminated soil. Five different treatments, including natural attenuation (NA), biostimulation (BS), biosurfactant addition (BE), bioaugmentation (BA), and a combined treatment (CT) of biostimulation, biosurfactant addition, and bioaugmentation, were used to analyze the degradation rate and microbial communities. After 120 days, the level of remaining hydrocarbons after all the treatments was similar. however, the highest rate (k) of total petroleum hydrocarbon (TPH) degradation was observed with the CT treatment (P < 0.05). The total bacterial counts increased during the first 2 weeks with all the treatments, and then remained stable. The bacterial communities and alkane monooxygenase gene fragment, alkB, were compared by denaturing gradient gel electrophoresis (DGGE). The DGGE analyses of the BA and CT treatments, which included Nocardia sp. H17-1, revealed a simple dominant population structure, compared with the other treatments. The Shannon-Weaver diversity index (H) and Simpson dominance index (D), calculated from the DGGE profiles using 16S rDNA, showed considerable qualitative differences in the community structure before and after the bioremediation treatment as,,well as between treatment conditions.
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자연과학대학 > 생명과학전공 > Journal papers
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