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Microfluidic immunoassay platform using antibody-immobilized glass beads and its application for detection of Escherichia coli O157 : H7

Title
Microfluidic immunoassay platform using antibody-immobilized glass beads and its application for detection of Escherichia coli O157 : H7
Authors
Lee, NYYang, YKim, YSPark, S
Ewha Authors
박성수
Issue Date
2006
Journal Title
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
ISSN
0253-2964JCR Link
Citation
vol. 27, no. 4, pp. 479 - 483
Keywords
microchamberglass headIgG immobilizationpathomilc bacteriaEscherichia coli O157 : H17
Publisher
KOREAN CHEMICAL SOC
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
We developed a microfluidic immunoassay platform for the decision of various analytes such as bacterial pathogen by packing antibody-immobilized glass beads in spatially-isolated microchambers on a microfludic device. Primary amines of antibody were covalently conjugated to carboxyl-terminated glass beads previously treated with aminosilane followed by glutaraldehyde. Through this covalent binding, up to 905 mu g immunoglobulin G (IgG) per gram of glass beads was immobilized. For application, glass beads attaching antibody specific to Escherichia coli O157:H7, a foodborne pathogen, were packed into a microfluidic device and used for the detection of the serotype. This prototype immunoassay device can be used for the simulataneous detection of multiple analytes by sequentially packing different-sized glass beads attaching different antibody in discrete microchambers on a single microfluidic device.
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자연과학대학 > 화학·나노과학전공 > Journal papers
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