Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 하헌주 | * |
dc.date.accessioned | 2016-08-27T02:08:17Z | - |
dc.date.available | 2016-08-27T02:08:17Z | - |
dc.date.issued | 2005 | * |
dc.identifier.issn | 0896-8608 | * |
dc.identifier.other | OAK-2628 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/215882 | - |
dc.description.abstract | Objective: To demonstrate the presence of an independent renin-angiotensin system (RAS) in the peritoneum and to determine the role of locally produced angiotensin (Ang) II in high gLucose-induced upregulation of transforming growth factor (TGF)-beta 1 and fibronectin by human peritoneal mesothelial cells (HPMC). Methods: In cultured HPMC, the expression of mRNAs for angiotensinogen, angiotensin-converting enzyme (ACE), Ang II type 1 receptor (AT1), and TGF-beta 1 was evaluated by real-time polymerase chain reaction; ACE, AT1, and fibronectin proteins by Western blot analysis; and Ang 1, Ang II, and TGF-beta 1 proteins by ELISA. Dichlorofluorscein (DCF)-sensitive cellular reactive oxygen species (ROS) were measured by fluorometry. Results: HPMC constitutively expressed all the components of RAS, and 50 mmoL/L D-glucose (high glucose) significantly increased angiotensinogen, ACE, and AT1 m RNAs and ACE, AT1, and Ang II proteins. Ang 11 increased TGF-beta 1 and fibronectin protein expression and DCF-sensitive cellular ROS. Losartan prevented Ang II-induced increase in cellular ROS. Both Losartan and captopril inhibited high gLucose-induced upregulation of TGF-beta 1 and fibronectin expression in HPMC in a dose-dependent manner. Antioxidant catalase and NADPH oxidase inhibitor diphenyleneiodinium effectively inhibited Ang 11-induced TGF-beta 1 and fibronectin protein expression. Conclusions: The present data demonstrate that HPMC constitutively express RAS, that Ang II produced by HPMC mediates high gLucose-induced upregulation of TGF-beta 1 and fibronectin expression, and that Ang II-induced TGF-beta 1 and fibronectin expression in HPMC is mediated by NADPH oxidase-dependent ROS. These data suggest that Locally produced Ang II and ROS in the peritoneum may be potential therapeutic targets in peritoneal fibrosis during long-term peritoneal dialysis. | * |
dc.language | English | * |
dc.publisher | MULTIMED INC | * |
dc.subject | high glucose | * |
dc.subject | fibronectin | * |
dc.subject | peritoneal fibrosis | * |
dc.subject | reactive oxygen species | * |
dc.subject | renin-angiotensin system | * |
dc.subject | TGF-beta 1 | * |
dc.title | Angiotensin II mediates high glucose-induced TGF-beta 1 and fibronectin upregulation in HPMC through reactive oxygen species | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 25 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 38 | * |
dc.relation.lastpage | 47 | * |
dc.relation.journaltitle | PERITONEAL DIALYSIS INTERNATIONAL | * |
dc.identifier.wosid | WOS:000227628400011 | * |
dc.identifier.scopusid | 2-s2.0-15044366331 | * |
dc.author.google | Noh, H | * |
dc.author.google | Ha, H | * |
dc.author.google | Yu, MR | * |
dc.author.google | Kim, YO | * |
dc.author.google | Kim, JH | * |
dc.author.google | Lee, HB | * |
dc.contributor.scopusid | 하헌주(7202277106) | * |
dc.date.modifydate | 20240422113229 | * |