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Probing the caspase-3 active site by fluorescence lifetime measurements

Title
Probing the caspase-3 active site by fluorescence lifetime measurements
Authors
Kyoung, MKim, SYSeok, HYPark, ISLee, MY
Ewha Authors
이민영
SCOPUS Author ID
이민영scopus
Issue Date
2002
Journal Title
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
ISSN
1570-9639JCR Link
Citation
vol. 1598, no. 1-2, pp. 74 - 79
Keywords
caspase-3active sitefluorescence lifetimetryptophaninhibitoranisotropy
Publisher
ELSEVIER SCIENCE BV
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The active site of an apoptotic enzyme caspase-3 was characterized by measuring the intrinsic fluorescence of two tryptophan residues. Temperature dependence of the intrinsic fluorescence, the energy homotransfer between the tryptophan residues, and the fluorescence quenching by tetrapeptide inhibitors were investigated by the fluorescence lifetime measurements. It has been observed that the fluorescence lifetimes of caspase-3 in complex with inhibitors were significantly shortened by the electron transfer process. (C) 2002 Elsevier Science B.V All rights reserved.
DOI
10.1016/S0167-4838(02)00333-3
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자연과학대학 > 화학·나노과학전공 > Journal papers
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