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Human kinesin superfamily member 4 is dominantly localized in the nuclear matrix and is associated with chromosomes during mitosis

Title
Human kinesin superfamily member 4 is dominantly localized in the nuclear matrix and is associated with chromosomes during mitosis
Authors
Lee, YMLee, SLee, EShin, HHahn, HChoi, WKim, W
Ewha Authors
최원자
SCOPUS Author ID
최원자scopus
Issue Date
2001
Journal Title
BIOCHEMICAL JOURNAL
ISSN
0264-6021JCR Link
Citation
vol. 360, pp. 549 - 556
Keywords
chromosomal associationKIF4 motor proteinnuclear localization signal
Publisher
PORTLAND PRESS
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
In a previous study, we identified the human counterpart of murine kinesin superfamily member 4 (KIF4), a microtubule-based motor protein [Oh, Hahn, Torrey, Shin, Choi, Lee, Morse and Kim (2000) Biochim. Biophys. Acta 1493, 219 224]. As an initial step to understand the function(s) of human KIF4, its subcellular localization in HeLa cells was examined by using immunocytochemical and subcellular fractionation methods, and it was found that most KIF4 is localized in the nucleus. Since murine KIF4 is known to transport cytoplasmic vesicles, dominant nuclear localization of the human counterpart was somewhat surprising. Subsequent subnuclear fractionation revealed predominant association of KIF4 with the nuclear matrix. These results clearly indicate that human KIF4 is, at least, a nuclear protein. In further confirmation of this conclusion, the hexapeptide PKLRRR (amino acids 773-778) in the molecule was found to function as a nuclear localization signal. During the mitotic phase of the cell cycle, human KIF4 was associated with the chromosomes, suggesting that human KIF4 might be a microtubule-based mitotic motor, with DNA as its cargo.
DOI
10.1042/0264-6021:3600549
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자연과학대학 > 생명과학전공 > Journal papers
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