Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 배윤수 | * |
dc.date.accessioned | 2016-08-27T02:08:28Z | - |
dc.date.available | 2016-08-27T02:08:28Z | - |
dc.date.issued | 2000 | * |
dc.identifier.issn | 0022-1767 | * |
dc.identifier.other | OAK-478 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/215363 | - |
dc.description.abstract | Stimulation of human lung fibroblast cells with TGF-beta 1 resulted in a transient burst of reactive oxygen species with maximal increase at 5 min after treatment. This reactive oxygen species increase was inhibited by the antioxidant, N-acetyl-L-cysteine (NAC), TGF-beta 1 treatment stimulated IL-6 gene expression and protein synthesis in human lung fibroblast cells. Antioxidants including NAC, glutathione, and catalase reduced TGF-beta 1-induced IL-6 gene expression, and direct H2O2 treatment induced IL-6 expression in a dose-dependent manner. NAC also reduced TGF-beta 1-induced AP-1 binding activity, which is involved in IL-6 gene expression. It has been reported that Ca2+ influx is stimulated by TGF-beta 1 treatment. EGTA suppressed TGF-beta 1- or H2O2-induced IL-6 expression, and ionomycin increased IL-6 expression, with simultaneously modulating AP-1 activity in the same pattern. PD98059, an inhibitor of mitogen-activated protein kinase (MAPK) kinase/extracellular signal-related kinase kinase 1, suppressed TGF-beta 1- or H2O2-induced IL-6 and AP-1 activation. In addition, TGF-beta 1 or H2O2 increased MAPK activity which was reduced by EGTA and NAG, suggesting that MAPK is involved in TGF-beta 1-induced IL-6 expression. Taken together, these results indicate that TGF-beta 1 induces a transient increase of intracellular H2O2 production, which regulates downstream events such as Ca2+ influx, MAPK, and AP-1 activation and IL-6 gene expression. | * |
dc.language | English | * |
dc.publisher | AMER ASSOC IMMUNOLOGISTS | * |
dc.title | Requirement of hydrogen peroxide generation in TGF-beta 1 signal transduction in human lung fibroblast cells: Involvement of hydrogen peroxide and Ca2+ in TGF-beta 1-induced IL-6 expression | * |
dc.type | Article | * |
dc.relation.issue | 4 | * |
dc.relation.volume | 165 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 2190 | * |
dc.relation.lastpage | 2197 | * |
dc.relation.journaltitle | JOURNAL OF IMMUNOLOGY | * |
dc.identifier.wosid | WOS:000088626200060 | * |
dc.author.google | Junn, E | * |
dc.author.google | Lee, KN | * |
dc.author.google | Ju, HR | * |
dc.author.google | Han, SH | * |
dc.author.google | Im, JY | * |
dc.author.google | Kang, HS | * |
dc.author.google | Lee, TH | * |
dc.author.google | Bae, YS | * |
dc.author.google | Ha, KS | * |
dc.author.google | Lee, ZW | * |
dc.author.google | Rhee, SG | * |
dc.author.google | Choi, I | * |
dc.contributor.scopusid | 배윤수(15031067200) | * |
dc.date.modifydate | 20240415133331 | * |