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dc.contributor.advisor황은숙-
dc.contributor.author임지혜-
dc.creator임지혜-
dc.date.accessioned2024-08-19T16:31:08Z-
dc.date.available2024-08-19T16:31:08Z-
dc.date.issued2024-
dc.identifier.otherOAK-000000232682-
dc.identifier.urihttps://dcollection.ewha.ac.kr/common/orgView/000000232682en_US
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/268673-
dc.description.abstract아데노신은 면역 세포 활성화를 억제하는 신호 분자로 작용합니다. 자연적으로 발생하는 코디세핀은 동충하초에서 많이 발견되며, 아데노신과 유사한 활성을 가지고 있어 면역 기능을 강화하고 항암 활성을 나타내는 것으로 보고되었습니다. 이 연구는 RAW264.7 대식세포 세포주와 쥐 골수 유래 대식세포를 사용하여 코디세핀과 아데노신이 대식세포 활성에 미치는 영향을 비교하는 것을 목표로 합니다. 코디세핀은 아데노신 수용체를 경유하여 농도 의존적으로 염증성 사이토카인의 생성을 증가시킨 반면, 아데노신은 유의미한 영향을 미치지 않았습니다. 흥미롭게도 코디세핀은 주요 염증 매개체인 NF-κB의 인산화와 핵 내 이동을 촉진한 반면, 아데노신은 NF-κB 활성에 영향을 미치지 않았습니다. 추가 분석 결과, 코디세핀은 아데노신이 아닌 대식세포의 식세포 활성을 증가시켰습니다. 또한, 코디세핀 처리된 대식세포는 멜라노마 암세포로 더 잘 이동하였으며, 멜라노마 세포의 살상 효과가 더 잘 나타났습니다. 이는 코디세핀이 멜라노마 암세포 치료제로서의 잠재력이 있음을 시사합니다.;Adenosine acts as a signaling molecule that inhibits immune cell activation. Naturally occurring cordycepin, commonly found in Cordyceps, has been reported to have similar activity to adenosine, enhancing immune function and exhibiting anticancer activity. This study aims to compare the effects of cordycepin and adenosine on macrophage activity using the RAW264.7 macrophage cell line and mouse bone marrow-derived macrophages. Cordycepin increased the production of inflammatory cytokines in a dose-dependent manner via adenosine receptors, whereas adenosine had no significant effect. Interestingly, cordycepin enhanced the phosphorylation and nuclear translocation of NF-κB, a key inflammatory mediator, while adenosine did not affect NF-κB activity. Further analysis revealed that cordycepin, not adenosine, increased macrophage phagocytic activity. Additionally, macrophages treated with cordycepin exhibited enhanced migration towards melanoma cancer cells and showed a greater killing effect on these cells. This suggests that cordycepin has potential as a therapeutic agent for melanoma cancer cell therapy.-
dc.description.tableofcontentsI. Introduction 1 1. Functional Differences between Adenosine and Cordycepin in the Immune System 1 2. Macrophage polarization and phagocytosis 3 3. NF-κB signaling in macrophage 6 4. Adenosine receptor in macrophage 8 5. The risks of melanoma and the Need for Diverse Treatment approaches 10 6. Aim of this study 12 II. Materials and methods 13 1. Antibodies 13 2. Reagents 15 3. Mice 17 4. Cell culture 18 5. Bone marrow-derived macrophage culture 19 6. Immunoblot 20 7. Immunofluorescence 21 8. Reverse transcription and real-time PCR 22 9. Cytotoxicity assay 24 10. Reporter gene assay 25 11. Phagocytosis assay 26 III. Results 27 1. Cytotoxic effect of cordycepin and adenosine in macrophage 27 2. Cordycepin enhances the release of pro-inflammatory cytokines via NF-κB signaling, while adenosine does not 29 3. Cordycepin enhances the phagocytosis of macrophages 34 4. Cordycepin mediates its effects via the A2A adenosine receptor 36 5. Cordycepin-Treated Macrophages Exhibit Enhanced Phagocytosis of Melanoma Cells 38 6. Cordycepin-treated macrophages significantly decrease the viability of melanoma cells 40 IV. Discussion 42 V. References 44 VI. Abstract (in Korean) 49-
dc.formatapplication/pdf-
dc.format.extent2671183 bytes-
dc.languageeng-
dc.publisher이화여자대학교 대학원-
dc.subjectcordycepin, adenosine, macrophage-
dc.subject.ddc600-
dc.titleCordycepin, an adenosine analogue, shows different activity from adenosine in activating macrophage proliferation and phagocytosis-
dc.typeMaster's Thesis-
dc.format.pageviii, 49 p.-
dc.identifier.thesisdegreeMaster-
dc.identifier.major대학원 약학과-
dc.date.awarded2024. 8-
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