View : 170 Download: 0

Toxicity of simultaneous intrastromal and intracameral injection of voriconazole on corneal endothelium in a rabbit model

Title
Toxicity of simultaneous intrastromal and intracameral injection of voriconazole on corneal endothelium in a rabbit model
Authors
Moon, Ji YoungSuh, WoolJun, Roo MinHan, Kyung Eun
Ewha Authors
전루민한경은서울
SCOPUS Author ID
전루민scopus; 한경은scopus; 서울scopus
Issue Date
2023
Journal Title
CUTANEOUS AND OCULAR TOXICOLOGY
ISSN
1556-9527JCR Link

1556-9535JCR Link
Citation
CUTANEOUS AND OCULAR TOXICOLOGY vol. 42, no. 3, pp. 144 - 150
Keywords
Voriconazolecorneal endotheliumfungal keratitisintracameralintrastromal
Publisher
TAYLOR &

FRANCIS LTD
Indexed
SCIE; SCOPUS WOS
Document Type
Article
Abstract
PurposeTo investigate the toxicity of repeated simultaneous intrastromal and intracameral injections of voriconazole in corneal endothelial cells in a rabbit model.MethodsThirty-six eyes of 18 New Zealand white rabbits (six eyes per group) were divided into 6 groups according to the concentration of voriconazole (Group A, 0%; Group B, 0.05%; Group C, 0.1%; Group D, 0.25%; Group E, 0.5%; Group F, 1%). A combination of intrastromal and intracameral voriconazole injections were administrated to the eyes of each group three times on days 0, 3, and 7. Corneal clouding grades and central corneal thickness (CCT) were examined on days 0, 3, 7, 10, and 14. The endothelial cell counts (ECC) were measured on days 0 and 14. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were performed on day 14.ResultsGroup F (1%) showed more severe corneal clouding than the other groups (Groups A-E) from day 7 (p < 0.05, respectively). There were no significant differences in CCT and ECC among the six groups at any time point (p > 0.05, respectively). SEM revealed blurring of the cell border and loss of microvilli at concentrations & GE;0.25% (Groups D-F). TEM revealed microstructural changes in endothelial cells at concentrations & GE;0.1% (Groups C-F), and multiple vacuoles were observed at a concentration of 1% voriconazole (Group F).ConclusionsRepeated simultaneous intrastromal and intracameral voriconazole injections at a concentration of 0.1% or higher induced microstructural endothelial damage in rabbit corneal endothelial cells.
DOI
10.1080/15569527.2023.2226714|http://dx.doi.org/10.1080/15569527.2023.2226714
Appears in Collections:
의과대학 > 의학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

BROWSE