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Encyclopedia of Family A DNA Polymerases Localized in Organelles: Evolutionary Contribution of Bacteria Including the Proto-Mitochondrion

Title
Encyclopedia of Family A DNA Polymerases Localized in Organelles: Evolutionary Contribution of Bacteria Including the Proto-Mitochondrion
Authors
HaradaRyoHirakawaYoshihisaYabukiAkinoriKimEunsooYazakiEukiKamikawaRyomaNakanoKentaroEliášMarekInagakiYuji
Ewha Authors
김은수
SCOPUS Author ID
김은수scopus
Issue Date
2024
Journal Title
Molecular Biology and Evolution
ISSN
0737-4038JCR Link
Citation
Molecular Biology and Evolution vol. 41, no. 2
Keywords
DNA polymeraseendosymbiosislast eukaryotic common ancestorlateral gene transfermitochondriaplastids
Publisher
Oxford University Press
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
DNA polymerases synthesize DNA from deoxyribonucleotides in a semiconservative manner and serve as the core of DNA replication and repair machinery. In eukaryotic cells, there are 2 genome-containing organelles, mitochondria, and plastids, which were derived from an alphaproteobacterium and a cyanobacterium, respectively. Except for rare cases of genome-lacking mitochondria and plastids, both organelles must be served by nucleus-encoded DNA polymerases that localize and work in them to maintain their genomes. The evolution of organellar DNA polymerases has yet to be fully understood because of 2 unsettled issues. First, the diversity of organellar DNA polymerases has not been elucidated in the full spectrum of eukaryotes. Second, it is unclear when the DNA polymerases that were used originally in the endosymbiotic bacteria giving rise to mitochondria and plastids were discarded, as the organellar DNA polymerases known to date show no phylogenetic affinity to those of the extant alphaproteobacteria or cyanobacteria. In this study, we identified from diverse eukaryotes 134 family A DNA polymerase sequences, which were classified into 10 novel types, and explored their evolutionary origins. The subcellular localizations of selected DNA polymerases were further examined experimentally. The results presented here suggest that the diversity of organellar DNA polymerases has been shaped by multiple transfers of the PolI gene from phylogenetically broad bacteria, and their occurrence in eukaryotes was additionally impacted by secondary plastid endosymbioses. Finally, we propose that the last eukaryotic common ancestor may have possessed 2 mitochondrial DNA polymerases, POP, and a candidate of the direct descendant of the proto-mitochondrial DNA polymerase I, rdxPolA, identified in this study. © The Author(s) 2024.
DOI
10.1093/molbev/msae014
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자연과학대학 > 생명과학전공 > Journal papers
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