View : 202 Download: 0
Aspirin Challenge–Induced Genome-Wide DNA Methylation Profile of Peripheral Blood Lymphocytes in Aspirin-Exacerbated Respiratory Disease
- Title
- Aspirin Challenge–Induced Genome-Wide DNA Methylation Profile of Peripheral Blood Lymphocytes in Aspirin-Exacerbated Respiratory Disease
- Authors
- Lee; Jong-Uk; Chang; Hun Soo; Shim; Ji-Su; Kim; Min-Hye; Cho; Young-Joo; Min Kyung; Park; Seung-Lee; Sun Ju; Jong-Sook; Choon-Sik
- Ewha Authors
- 조영주; 김민혜; 심지수
- SCOPUS Author ID
- 조영주; 김민혜; 심지수
- Issue Date
- 2024
- Journal Title
- DNA and Cell Biology
- ISSN
- 1044-5498
- Citation
- DNA and Cell Biology vol. 43, no. 3, pp. 132 - 140
- Keywords
- aspirin-exacerbated respiratory disease; DNA CpG methylation; Introduction; lymphocytes
- Publisher
- Mary Ann Liebert Inc.
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Genetic variation and epigenetic factors are thought to contribute to the development of hypersensitivity to aspirin. DNA methylation fluctuates dynamically throughout the day. To discover new CpG methylation in lymphocytes associated with aspirin-exacerbated respiratory disease (AERD), we evaluated changes in global CpG methylation profiles from before to after an oral aspirin challenge in patients with AERD and aspirin-tolerant asthma (ATA). Whole-genome CpG methylation levels of peripheral blood mononuclear cells were quantified with an Illumina 860K Infinium Methylation EPIC BeadChip array and then adjusted for inferred lymphocyte fraction (ILF) with GLINT and Tensor Composition Analysis. Among the 866,091 CpGs in the array, differentially methylated CpGs (DMCs) were found in 6 CpGs in samples from all 12 patients with asthma included in the study (AERD, n = 6; ATA, n = 6). DMCs were found in 3 CpGs in the 6 ATA samples and in 615 CpGs in the 6 AERD samples. A total of 663 DMCs in 415 genes and 214 intergenic regions differed significantly in the AERD compared with the ATA. In promoters, 126 CpG loci were predicted to bind to 38 transcription factors (TFs), many of which were factors already known to be involved in the pathogenesis of asthma and immune responses. In conclusion, we identified 615 new CpGs methylated in peripheral blood lymphocytes by oral aspirin challenge in AERD but not in ATA. These findings indicate that oral aspirin challenge induces epigenetic changes in ILFs, specifically in AERD patients, possibly via changes in TF binding, which may have epigenetic effects on the development of AERD. © Mary Ann Liebert, Inc.
- DOI
- 10.1089/dna.2023.0218
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML