View : 101 Download: 0

Asuc_0142 of <i>Actinobacillus succinogenes</i> 130Z is the l-aspartate/ C4-dicarboxylate exchanger DcuA

Title
Asuc_0142 of <i>Actinobacillus succinogenes</i> 130Z is the l-aspartate/ C4-dicarboxylate exchanger DcuA
Authors
Cho, Young BinPark, Ji WonUnden, GottfriedKim, Ok Bin
Ewha Authors
김옥빈
SCOPUS Author ID
김옥빈scopus
Issue Date
2023
Journal Title
MICROBIOLOGY-SGM
ISSN
1350-0872JCR Link

1465-2080JCR Link
Citation
MICROBIOLOGY-SGM vol. 169, no. 10
Keywords
DcuAL-aspartatebovine rumenC4DCActinobacillus succinogenes
Publisher
MICROBIOLOGY SOC
Indexed
SCIE; SCOPUS WOS
Document Type
Article
Abstract
Anaerobic bacteria often use antiporters DcuB (malate/succinate antiport) or DcuA (l-aspartate/ succinate antiport) for the excretion of succinate during fumarate respiration. The rumen bacterium Actinobacillus succinogenes is able to produce large amounts of succinate by fumarate respiration, using the DcuB-type transporter DcuE for l-malate/ succinate antiport. Asuc_0142 was annotated as a second DcuB-type transporter. Deletion of Asuc_0142 decreased the uptake rate for l-[C-14] aspartate into A. succinogenes cells. Properties of transport by heterologously expressed Asuc_0142 were investigated in an Escherichia coli mutant deficient of anaerobic C4DC transporters. Expression of Asuc_0142 resulted in high uptake activity for l-[C-14]fumarate or l-[C-14]aspartate, but the former showed a strong competitive inhibition by l-aspartate. In E. coli loaded with l-[C-14]aspartate, [C-14]succinate or [C-14]fumarate, extracellular C4DCs initiated excretion of the intracellular substrates, with a preference for l-aspartate ex/succinate in or l-aspartate ex/fumarate in antiport. These findings indicate that Asuc_0142 represents a DcuA-type transporter for l-aspartate uptake and l-aspartate ex/C4DC in antiport, differentiating it from the DcuB-type transporter DcuE for l-malate ex/succinate in antiport. Sequence analysis and predicted structural characteristics confirm structural similarity of Asuc_0142 to DcuA, and Asuc_0142 was thus re-named as DcuA As. The bovine rumen fluid contains l-aspartate (99.6 mu M), whereas fumarate and l-malate are absent. Therefore, bovine rumen colonisers depend on l-aspartate as an exogenous substrate for fumarate respiration. A. succinogenes encodes HemG (protoporphyrinogen oxidase) and PyrD (dihydroorotate dehydrogenase) for haem and pyrimidine biosynthesis. The enzymes require fumarate as an electron acceptor, suggesting an essential role for l-aspartate, DcuA As, and fumarate respiration for A. succinogenes growing in the bovine rumen.
DOI
10.1099/mic.0.001411|http://dx.doi.org/10.1099/mic.0.001411
Appears in Collections:
자연과학대학 > 생명과학전공 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

BROWSE