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Control of the signaling role of PtdIns(4)P at the plasma membrane through H2O2-dependent inactivation of synaptojanin 2 during endocytosis
- Title
- Control of the signaling role of PtdIns(4)P at the plasma membrane through H2O2-dependent inactivation of synaptojanin 2 during endocytosis
- Authors
- Jo; Su In; Kim; Suree; Lim; Jung Mi; Rhee; Sue Goo; Jeong; Bo-Gyeong; Cha; Sun-Shin; Chang; Jae-Byum; Kang; Dongmin
- Ewha Authors
- 강동민; 김수리
- SCOPUS Author ID
- 강동민
- Issue Date
- 2024
- Journal Title
- Redox Biology
- ISSN
- 2213-2317
- Citation
- Redox Biology vol. 71
- Keywords
- Endocytosis; Hydrogen peroxide; Phosphatidylinositol 4-phosphate [PtdIns(4)P]; Protein oxidation; Synaptojanin (synj)
- Publisher
- Elsevier B.V.
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] is implicated in various processes, including hormone-induced signal transduction, endocytosis, and exocytosis in the plasma membrane. However, how H2O2 accumulation regulates the levels of PtdIns(4,5)P2 in the plasma membrane in cells stimulated with epidermal growth factors (EGFs) is not known. We show that a plasma membrane PtdIns(4,5)P2-degrading enzyme, synaptojanin (Synj) phosphatase, is inactivated through oxidation by H2O2. Intriguingly, H2O2 inhibits the 4-phosphatase activity of Synj but not the 5-phosphatase activity. In EGF-activated cells, the oxidation of Synj dual phosphatase is required for the transient increase in the plasma membrane levels of phosphatidylinositol 4-phosphate [PtdIns(4)P], which can control EGF receptor-mediated endocytosis. These results indicate that intracellular H2O2 molecules act as signaling mediators to fine-tune endocytosis by controlling the stability of plasma membrane PtdIns(4)P, an intermediate product of Synj phosphoinositide dual phosphatase. © 2024 The Authors
- DOI
- 10.1016/j.redox.2024.103097
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
- Files in This Item:
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1-s2.0-S2213231724000739-main.pdf(11.06 MB)
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